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Authors
Yu, TianxiongKoppetsch, Birgit S.
Pagliarani, Sara
Johnston, Stephen
Silverstein, Noah J.
Luban, Jeremy
Chappell, Keith
Weng, Zhiping
Theurkauf, William E.
UMass Chan Affiliations
Graduate School of Biomedical SciencesProgram in Molecular Medicine
Program in Bioinformatics and Integrative Biology
Document Type
Journal ArticlePublication Date
2019-10-10Keywords
AKVKoRV-A
genome invasion
germ-line genome
koala
piRNA
piRNA clusters
retrovirus
Biochemistry, Biophysics, and Structural Biology
Computational Biology
Genetic Phenomena
Genetics and Genomics
Hemic and Immune Systems
Nucleic Acids, Nucleotides, and Nucleosides
Metadata
Show full item recordAbstract
Antisense Piwi-interacting RNAs (piRNAs) guide silencing of established transposons during germline development, and sense piRNAs drive ping-pong amplification of the antisense pool, but how the germline responds to genome invasion is not understood. The KoRV-A gammaretrovirus infects the soma and germline and is sweeping through wild koalas by a combination of horizontal and vertical transfer, allowing direct analysis of retroviral invasion of the germline genome. Gammaretroviruses produce spliced Env mRNAs and unspliced transcripts encoding Gag, Pol, and the viral genome, but KoRV-A piRNAs are almost exclusively derived from unspliced genomic transcripts and are strongly sense-strand biased. Significantly, selective piRNA processing of unspliced proviral transcripts is conserved from insects to placental mammals. We speculate that bypassed splicing generates a conserved molecular pattern that directs proviral genomic transcripts to the piRNA biogenesis machinery and that this "innate" piRNA response suppresses transposition until antisense piRNAs are produced, establishing sequence-specific adaptive immunity.Source
Cell. 2019 Oct 17;179(3):632-643.e12. doi: 10.1016/j.cell.2019.09.002. Epub 2019 Oct 10. Link to article on publisher's site
DOI
10.1016/j.cell.2019.09.002Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25865PubMed ID
31607510Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.cell.2019.09.002