UMMS Affiliation
Program in Molecular Medicine; Program in Bioinformatics and Integrative Biology
Publication Date
2018-01-22
Document Type
Article
Disciplines
Bacterial Infections and Mycoses | Bioinformatics | Computational Biology | Genetics and Genomics | Immunity | Immunology of Infectious Disease
Abstract
To better understand the innate immune response to Vibrio cholerae infection, we tracked gene expression in the duodenal mucosa of 11 Bangladeshi adults with cholera, using biopsy specimens obtained immediately after rehydration and 30 and 180 days later. We identified differentially expressed genes and performed an analysis to predict differentially regulated pathways and upstream regulators. During acute cholera, there was a broad increase in the expression of genes associated with innate immunity, including activation of the NF-kappaB, mitogen-activated protein kinase (MAPK), and Toll-like receptor (TLR)-mediated signaling pathways, which, unexpectedly, persisted even 30 days after infection. Focusing on early differences in gene expression, we identified 37 genes that were differentially expressed on days 2 and 30 across the 11 participants. These genes included the endosomal Toll-like receptor gene TLR8, which was expressed in lamina propria cells. Underscoring a potential role for endosomal TLR-mediated signaling in vivo, our pathway analysis found that interferon regulatory factor 7 and beta 1 and alpha 2 interferons were among the top upstream regulators activated during cholera. Among the innate immune effectors, we found that the gene for DUOX2, an NADPH oxidase involved in the maintenance of intestinal homeostasis, was upregulated in intestinal epithelial cells during cholera. Notably, the observed increases in DUOX2 and TLR8 expression were also modeled in vitro when Caco-2 or THP-1 cells, respectively, were stimulated with live V. cholerae but not with heat-killed organisms or cholera toxin alone. These previously unidentified features of the innate immune response to V. cholerae extend our understanding of the mucosal immune signaling pathways and effectors activated in vivo following cholera.
Keywords
Vibrio cholerae, immune mechanisms, mucosal immunity
Rights and Permissions
Copyright © 2018, American Society for Microbiology. Publisher PDF posted as allowed by the publisher's copyright policy at https://journals.asm.org/content/copyright-transfer-and-supplemental-material-license-agreement-2017.
DOI of Published Version
10.1128/IAI.00594-17
Source
Infect Immun. 2018 Jan 22;86(2). pii: e00594-17. doi: 10.1128/IAI.00594-17. Print 2018 Feb. Link to article on publisher's site
Journal/Book/Conference Title
Infection and immunity
Related Resources
PubMed ID
29133347
Repository Citation
Bourque, Daniel L.; Genereux, Diane P.; Karlsson, Elinor K.; Qadri, Firdausi; and Harris, Jason B., "Analysis of the Human Mucosal Response to Cholera Reveals Sustained Activation of Innate Immune Signaling Pathways" (2018). Program in Bioinformatics and Integrative Biology Publications and Presentations. 140.
https://escholarship.umassmed.edu/bioinformatics_pubs/140
Included in
Bacterial Infections and Mycoses Commons, Bioinformatics Commons, Computational Biology Commons, Immunity Commons, Immunology of Infectious Disease Commons
Comments
Full author list omitted for brevity. For the full list of authors, see article.