Program in Bioinformatics and Integrative Biology
Amino Acids, Peptides, and Proteins | Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Genetic Phenomena | Genetics | Genomics | Integrative Biology | Nucleic Acids, Nucleotides, and Nucleosides
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
C. elegans, chromosomes, gene expression, genetics, genomics
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DOI of Published Version
Elife. 2018 Dec 21;7. pii: 35478. doi: 10.7554/eLife.35478. [Epub ahead of print] Link to article on publisher's site
Davis GM, Tu S, Wilce JA, Claycomb JM, Weng Z, Boag PR. (2018). The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans. Program in Bioinformatics and Integrative Biology Publications. https://doi.org/10.7554/eLife.35478. Retrieved from https://escholarship.umassmed.edu/bioinformatics_pubs/139
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