Program in Bioinformatics and Integrative Biology
Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Integrative Biology | Systems Biology
pirScan is a web-based tool for identifying C. elegans piRNA-targeting sites within a given mRNA or spliced DNA sequence. The purpose of our tool is to allow C. elegans researchers to predict piRNA targeting sites and to avoid the persistent germline silencing of transgenes that has rendered many constructs unusable. pirScan fulfills this purpose by first enumerating the predicted piRNA-targeting sites present in an input sequence. This prediction can be exported in a tabular or graphical format. Subsequently, pirScan suggests silent mutations that can be introduced to the input sequence that would allow the modified transgene to avoid piRNA targeting. The user can customize the piRNA targeting stringency and the silent mutations that he/she wants to introduce into the sequence. The modified sequences can be re-submitted to be certain that any previously present piRNA-targeting sites are now absent and no new piRNA-targeting sites are accidentally generated. This revised sequence can finally be downloaded as a text file and/or visualized in a graphical format. pirScan is freely available for academic use at http://cosbi4.ee.ncku.edu.tw/pirScan/.
PIWI-interacting RNAs, piRNAs, targeting sites, tools
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Copyright © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI of Published Version
Nucleic Acids Res. 2018 Jul 2;46(W1):W43-W48. doi: 10.1093/nar/gky277. Link to article on publisher's site
Nucleic acids research
Wu W, Huang W, Brown JS, Zhang D, Song X, Chen H, Tu S, Weng Z, Lee H. (2018). pirScan: a webserver to predict piRNA targeting sites and to avoid transgene silencing in C. elegans. Program in Bioinformatics and Integrative Biology Publications and Presentations. https://doi.org/10.1093/nar/gky277. Retrieved from https://escholarship.umassmed.edu/bioinformatics_pubs/137
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License