Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline
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Authors
Shen, En-ZhiChen, Hao
Ozturk, Ahmet R.
Tu, Shikui
Shirayama, Masaki
Tang, Wen
Ding, Yue-He
Dai, Siyuan
Weng, Zhiping
Mello, Craig C.
Student Authors
Siyuan DaiAcademic Program
InterdisciplinaryDocument Type
Journal ArticlePublication Date
2018-02-22
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Show full item recordAbstract
piRNAs (Piwi-interacting small RNAs) engage Piwi Argonautes to silence transposons and promote fertility in animal germlines. Genetic and computational studies have suggested that C. elegans piRNAs tolerate mismatched pairing and in principle could target every transcript. Here we employ in vivo cross-linking to identify transcriptome-wide interactions between piRNAs and target RNAs. We show that piRNAs engage all germline mRNAs and that piRNA binding follows microRNA-like pairing rules. Targeting correlates better with binding energy than with piRNA abundance, suggesting that piRNA concentration does not limit targeting. In mRNAs silenced by piRNAs, secondary small RNAs accumulate at the center and ends of piRNA binding sites. In germline-expressed mRNAs, however, targeting by the CSR-1 Argonaute correlates with reduced piRNA binding density and suppression of piRNA-associated secondary small RNAs. Our findings reveal physiologically important and nuanced regulation of individual piRNA targets and provide evidence for a comprehensive post-transcriptional regulatory step in germline gene expression.Source
Shen EZ, Chen H, Ozturk AR, Tu S, Shirayama M, Tang W, Ding YH, Dai SY, Weng Z, Mello CC. Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline. Cell. 2018 Feb 22;172(5):937-951.e18. doi: 10.1016/j.cell.2018.02.002. Epub 2018 Feb 15. PMID: 29456082; PMCID: PMC5905434.DOI
10.1016/j.cell.2018.02.002Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25839PubMed ID
29456082ae974a485f413a2113503eed53cd6c53
10.1016/j.cell.2018.02.002