Title
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
UMMS Affiliation
RNA Therapeutics Institute; Program in Bioinformatics and Integrative Biology; Wellstone Muscular Dystrophy Program, Department of Neurology; Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology; Department of Molecular, Cell and Cancer Biology
Publication Date
2017-06-14
Document Type
Article
Disciplines
Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Integrative Biology | Systems Biology
Abstract
CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of beta-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.
Keywords
CRISPR/Cas9 genome editing, single-guide RNAs, sgRNAs, exon skipping
Rights and Permissions
Copyright © The Author(s). 2017.
DOI of Published Version
10.1186/s13059-017-1237-8
Source
Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. Link to article on publisher's site
Journal/Book/Conference Title
Genome biology
Related Resources
PubMed ID
28615073
Repository Citation
Mou, Haiwei; Smith, Jordan L.; Peng, Lingtao; Moore, Jill; Zhang, Xiao-Ou; Song, Chun-Qing; Sheel, Ankur; Ozata, Deniz M.; Li, Yingxiang; Emerson, Charles P. Jr.; Sontheimer, Erik J.; Moore, Melissa J.; Weng, Zhiping; and Xue, Wen, "CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion" (2017). Program in Bioinformatics and Integrative Biology Publications and Presentations. 111.
https://escholarship.umassmed.edu/bioinformatics_pubs/111
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Bioinformatics Commons, Computational Biology Commons, Integrative Biology Commons, Systems Biology Commons
Comments
Full author list omitted for brevity. For full list of authors see article.