Title

Brain-specific change in alternative splicing of Tau exon 6 in myotonic dystrophy type 1

UMMS Affiliation

Department of Cell Biology,Shriver Center

Publication Date

2-21-2006

Document Type

Article

Subjects

*Alternative Splicing; Brain; CCAAT-Enhancer-Binding Protein-delta; Cell Line; *Exons; Humans; Middle Aged; Muscle, Skeletal; Myotonic Dystrophy; Nerve Tissue Proteins; Neuroglia; Neurons; Protein-Serine-Threonine Kinases; RNA-Binding Proteins; tau Proteins

Disciplines

Cell Biology

Abstract

Alternative splicing is altered in myotonic dystrophy of type 1 (DM1), a syndrome caused by an increase of CTG triplet repeats in the 3' untranslated region of the myotonic dystrophy protein kinase gene. Previously, we reported the preferential skipping of Tau exon 2 in DM1 brains. In this study, we analyze the alternative splicing of Tau exon 6 which can be inserted in three different forms (c, p and d) depending on the 3' splice site used. In fact, inclusion of exon 6c decreases in DM1 brains compared to control brains whereas inclusion of 6d increases. Alteration of exon 6 splicing was not observed in DM1 muscle although this exon was inserted in RNAs from normal muscle and DM1 splicing alterations were first described in this organ. In contrast, alteration of exon 2 of Tau mRNA was observed in both muscle and brain. However, co-transfections of a minigene containing exon 6 with CELF or MBNL1 cDNAs, two splicing factor families suspected to be involved in DM1, showed that they influence exon 6 splicing. Altogether, these results show the importance of determining all the exons and organs targeted by mis-splicing to determine the dysregulation mechanisms of mis-splicing in DM1.

DOI of Published Version

10.1016/j.bbadis.2005.12.003

Source

Biochim Biophys Acta. 2006 Apr;1762(4):460-7. Epub 2005 Dec 29. Link to article on publisher's site

Journal/Book/Conference Title

Biochimica et biophysica acta

Related Resources

Link to Article in PubMed

PubMed ID

16487687

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