Saitohin, which is nested in the tau locus and confers allele-specific susceptibility to several neurodegenerative diseases, interacts with peroxiredoxin 6
Department of Cell Biology
Alleles; Animals; Base Sequence; COS Cells; Cell Line; Cercopithecus aethiops; DNA; Haplotypes; Heredodegenerative Disorders, Nervous; System; Humans; Peroxidases; Peroxiredoxin VI; Peroxiredoxins; Pick Disease of the Brain; RNA Splicing; Recombinant Fusion Proteins; Two-Hybrid System Techniques; tau Proteins
Saitohin is a gene unique to humans and their closest relatives, the function of which is not yet known. Saitohin contains a single polymorphism (Q7R), and its Q and R alleles belong to the H1 and H2 tau haplotype, respectively. The Saitohin Q allele confers susceptibility to several neurodegenerative diseases. To get a handle on Saitohin function, we used it as a bait in a yeast two-hybrid screen. By this assay and subsequent co-immunoprecipitation and glutathione S-transferase pull-down assays, we discovered and confirmed that Saitohin interacts with peroxiredoxin 6, a unique member of that family that is bifunctional and the levels of which increase in Pick disease. The strength of the interaction appeared to be allele-specific, giving the first distinction between the two forms of Saitohin.
DOI of Published Version
J Biol Chem. 2005 Nov 25;280(47):39268-72. Epub 2005 Sep 26. Link to article on publisher's site
The Journal of biological chemistry
Gao L, Tse S, Conrad C, Andreadis A. (2005). Saitohin, which is nested in the tau locus and confers allele-specific susceptibility to several neurodegenerative diseases, interacts with peroxiredoxin 6. Andreadis Lab Publications. https://doi.org/10.1074/jbc.M506116200. Retrieved from https://escholarship.umassmed.edu/andreadis/12