Disparate effects of telomere attrition on gene expression during replicative senescence of human mammary epithelial cells cultured under different conditions
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2004-08-15Keywords
AdultCell Aging
Cell Lineage
Epithelial Cells
Female
*Gene Expression Regulation
Humans
Mammary Glands, Animal
Oligonucleotide Array Sequence Analysis
*Telomere
Cell Biology
Metadata
Show full item recordAbstract
Telomere shortening in populations of human mammary epithelial cells (HMECs) that survive early replicative arrest (M0) by the inactivation of p16(INK4A) during cell culture on plastic dishes leads to a state of permanent replicative arrest termed senescence. While culture of HMECs on feeder layers abrogates M0 and p16(INK4A) inactivation, progressive telomere attrition in these cells also eventually results in permanent replicative arrest. Expression of telomerase prevents both senescence on plastic (S-P) and senescence on feeder layers (S-FL) in HMECs, as it does also in cultured primary human fibroblasts. We report here that the gene expression profiles of senescence in HMECs of the same lineage maintained under different culture conditions showed surprisingly little commonality. Moreover, neither of these senescence-associated profiles in HMECs resembles the profile for senescence in human fibroblasts. These results indicate that senescence-associated alterations in gene expression resulting from telomere attrition are affected by culture conditions as well as by cell origins, and argue that replicative senescence at the molecular level is a diverse rather than unique cellular process.Source
Oncogene. 2004 Aug 19;23(37):6193-8. Link to article on publisher's siteDOI
10.1038/sj.onc.1207834Permanent Link to this Item
http://hdl.handle.net/20.500.14038/51105PubMed ID
15195144Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/sj.onc.1207834