UMMS Affiliation

Department of Cell Biology; Department of Physiology; Program in Molecular Medicine

Date

12-30-2009

Document Type

Article

Medical Subject Headings

Algal Proteins; Axoneme; Chlamydomonas reinhardtii; Flagella; Protein Transport; Signal Transduction

Disciplines

Cell Biology

Abstract

In humans, seven evolutionarily conserved genes that cause the cilia-related disorder Bardet-Biedl syndrome (BBS) encode proteins that form a complex termed the BBSome. The function of the BBSome in the cilium is not well understood. We purified a BBSome-like complex from Chlamydomonas reinhardtii flagella and found that it contains at least BBS1, -4, -5, -7, and -8 and undergoes intraflagellar transport (IFT) in association with a subset of IFT particles. C. reinhardtii insertional mutants defective in BBS1, -4, and -7 assemble motile, full-length flagella but lack the ability to phototax. In the bbs4 mutant, the assembly and transport of IFT particles are unaffected, but the flagella abnormally accumulate several signaling proteins that may disrupt phototaxis. We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT.

Rights and Permissions

Citation: J Cell Biol. 2009 Dec 28;187(7):1117-32. Link to article on publisher's site

Related Resources

Link to Article in PubMed

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