This collection showcases the journal articles and other publications authored by researchers in the Witman Lab in the Department of Cell and Developmental Biology at the University of Massachusetts Medical School.

Submissions from 2013

Link

Isolation of Chlamydomonas flagella, Branch Craige, Jason M. Brown, and George B. Witman (Current Protocols in Cell Biology)

PDF

Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase, Karl-Ferdinand Lechtreck, Jason M. Brown, Julio L. Sampaio, Julie M. Craft, Andrej Shevchenko, James E. Evans, and George B. Witman (The Journal of cell biology)

PDF

Flagellar central pair assembly in Chlamydomonas reinhardtii, Karl-Ferdinand Lechtreck, Travis J. Gould, and George B. Witman (Cilia)

PDF

Avalanche-like behavior in ciliary import, William B. Ludington, Kimberly A. Wemmer, Karl-Ferdinand Lechtreck, George B. Witman, and Wallace F. Marshall (Proceedings of the National Academy of Sciences of the United States of America)

Submissions from 2012

Link

A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus, Jason Brown, Christen G. Dipetrillo, Elizabeth F. Smith, and George B. Witman (Journal of cell science)

PDF

The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function, Benjamin D. Engel, Hiroaki Ishikawa, Kimberly A. Wemmer, Stefan Geimer, Ken-ichi Wakabayashi, Masafumi Hirono, Branch Craige, Gregory J. Pazour, George B. Witman, Ritsu Kamiya, and Wallace F. Marshall (The Journal of cell biology)

Link

Dynein and Intraflagellar Transport, George B. Witman (Dyneins: Structure, Biology and Disease)

Submissions from 2011

Link

A unified taxonomy for ciliary dyneins, Erik F. Y. Hom, George B. Witman, Elizabeth H. Harris, Susan K. Dutcher, Ritsu Kamiya, David R. Mitchell, Gregory J. Pazour, Mary E. Porter, Winfield S. Sale, Maureen Wirschell, Toshiki Yagi, and Stephen M. King (Cytoskeleton (Hoboken))

PDF

Regulation of flagellar motility by the conserved flagellar protein CG34110/Ccdc135/FAP50, Yong Yang, Deborah A. Cochran, Mary D. Gargano, Iryna King, Nayef K. Samhat, Benjain P. Burger, Katherine R. Sabourin, Yuqing Hou, Junya Awata, David A.D. Parry, Wallace F. Marshall, George B. Witman, and Xiangyi Lu (Molecular biology of the cell)

Submissions from 2010

PDF

CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content, Branch Craige, Che-Chia Tsao, Dennis R. Diener, Yuqing Hou, Karl-Ferdinand Lechtreck, Joel L. Rosenbaum, and George B. Witman (The Journal of cell biology)

Submissions from 2009

Link

Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella, Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, and Wallace F. Marshall (Methods in cell biology)

PDF

The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella, Karl-Ferdinand Lechtreck, Eric C. Johnson, Tsuyoshi Sakai, Deborah A. Cochran, Bryan A. Ballif, John Rush, Gregory J. Pazour, Mitsuo Ikebe, and George B. Witman (The Journal of cell biology)

Link

HA-tagging of putative flagellar proteins in Chlamydomonas reinhardtii identifies a novel protein of intraflagellar transport complex B, Karl-Ferdinand Lechtreck, Scott Luro, Junya Awata, and George B. Witman (Cell motility and the cytoskeleton)

Link

High-speed digital imaging of ependymal cilia in the murine brain, Karl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman (Methods in cell biology)

Link

The Chlamydomonas Flagellum as a Model for Human Ciliary Disease, Gregory J. Pazour and George B. Witman (The Chlamydomonas Sourcebook)

PDF

IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding, Maureen Wirschell, Chun Yang, Pinfen Yang, Laura Fox, Haru-aki Yanagisawa, Ritsu Kamiya, George B. Witman, Mary E. Porter, and Winfield S. Sale (Molecular biology of the cell)

Submissions from 2008

Link

Mutations in Hydin impair ciliary motility in mice, Karl-Ferdinand Lechtreck, Philippe Delmotte, Michael L. Robinson, Michael J. Sanderson, and George B. Witman (The Journal of cell biology)

Submissions from 2007

PDF

Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella, Yuqing Hou, Hongmin Qin, John A. Follit, Gregory J. Pazour, Joel L. Rosenbaum, and George B. Witman (The Journal of cell biology)

Link

Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella, Kaiyao Huang, Dennis R. Diener, Aaron Mitchell, Gregory J. Pazour, George B. Witman, and Joel L. Rosenbaum (The Journal of cell biology)

PDF

Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility, Karl-Ferdinand Lechtreck and George B. Witman (The Journal of cell biology)

Link

The Chlamydomonas genome reveals the evolution of key animal and plant functions, Sabeeha S. Merchant, Simon E. Prochnik, George B. Witman, Gregory J. Pazour, Daniel S. Rokhsar, and Arthur R. Grossman (Science (New York, N.Y.))

Submissions from 2006

Link

Identification of predicted human outer dynein arm genes: candidates for primary ciliary dyskinesia genes, Gregory J. Pazour, Nathan S. Agrin, Bethany L. Walker, and George B. Witman (Journal of medical genetics)

Link

Radial spoke proteins of Chlamydomonas flagella, Pinfen Yang, Dennis R. Diener, Chun Yang, Takahiro Kohno, Gregory J. Pazour, Jennifer M. Dienes, Nathan S. Agrin, Stephen M. King, Winfield S. Sale, Ritsu Kamiya, Joel L. Rosenbaum, and George B. Witman (Journal of cell science)

Submissions from 2005

Link

Differential light chain assembly influences outer arm dynein motor function, Linda M. DiBella, Oksana Gorbatyuk, Miho Sakato, Ken-ichi Wakabayashi, Ramila S. Patel-King, Gregory J. Pazour, George B. Witman, and Stephen M. King (Molecular biology of the cell)

PDF

Proteomic analysis of a eukaryotic cilium, Gregory J. Pazour, Nathan S. Agrin, John D. Leszyk, and George B. Witman (The Journal of cell biology)

Link

Cytoplasmic dynein nomenclature, K. Kevin Pfister, Elizabeth M. C. Fisher, Ian R. Gibbons, Thomas S. Hays, Erika L. F. Holzbaur, J. Richard McIntosh, Mary E. Porter, Trina A. Schroer, Kevin T. Vaughan, George B. Witman, Stephen M. King, and Richard B. Vallee (The Journal of cell biology)

Submissions from 2004

Link

A dynein light intermediate chain, D1bLIC, is required for retrograde intraflagellar transport, Yuqing Hou, Gregory J. Pazour, and George B. Witman (Molecular biology of the cell)

Link

Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly, Agata Jurczyk, Adam Scott Gromley, Sambra D. Redick, Jovenal T. San Agustin, George B. Witman, Gregory J. Pazour, Dorien J. M. Peters, and Stephen J. Doxsey (The Journal of cell biology)

PDF

Novel role for a sterol response element binding protein in directing spermatogenic cell-specific gene expression, Hong Wang, Jovenal T. San Agustin, George B. Witman, and Daniel L. Kilpatrick (Molecular and cellular biology)

Link

The autosomal recessive polycystic kidney disease protein is localized to primary cilia, with concentration in the basal body area, Shixuan Wang, Ying Luo, Patricia D. Wilson, George B. Witman, and Jing Zhou (Journal of the American Society of Nephrology : JASN)

Link

Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase, Maureen Wirschell, Gregory J. Pazour, Akinori Yoda, Masafumi Hirono, Ritsu Kamiya, and George B. Witman (Molecular biology of the cell)

Submissions from 2003

Link

DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC, Diane M. Casey, Kazuo Inaba, Gregory J. Pazour, Saeko Takada, Ken-ichi Wakabayashi, Curtis G. Wilkerson, Ritsu Kamiya, and George B. Witman (Molecular biology of the cell)

Link

DC3, the smallest subunit of the Chlamydomonas flagellar outer dynein arm-docking complex, is a redox-sensitive calcium-binding protein, Diane M. Casey, Toshiki Yagi, Ritsu Kamiya, and George B. Witman (The Journal of biological chemistry)

Link

The vertebrate primary cilium is a sensory organelle, Gregory J. Pazour and George B. Witman (Current opinion in cell biology)

Link

Cell motility: deaf Drosophila keep the beat, George B. Witman (Current biology : CB)

Submissions from 2002

PDF

The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance, Gregory J. Pazour, Sheila A. Baker, James A. Deane, Douglas G. Cole, Bethany L. Dickert, Joel L. Rosenbaum, George B. Witman, and Joseph C. Besharse (The Journal of cell biology)

Link

Polycystin-2 localizes to kidney cilia and the ciliary level is elevated in orpk mice with polycystic kidney disease, Gregory J. Pazour, Jovenal T. San Agustin, John A. Follit, Joel L. Rosenbaum, and George B. Witman (Current biology : CB)

Link

Intraflagellar transport, Joel L. Rosenbaum and George B. Witman (Nature reviews. Molecular cell biology)

PDF

The outer dynein arm-docking complex: composition and characterization of a subunit (oda1) necessary for outer arm assembly, Saeko Takada, Curtis G. Wilkerson, Ken-ichi Wakabayashi, Ritsu Kamiya, and George B. Witman (Molecular biology of the cell)

Submissions from 2001

Link

Forward and reverse genetic analysis of microtubule motors in Chlamydomonas, Gregory J. Pazour and George B. Witman (Methods (San Diego, Calif.))

Link

Differential expression of the C(s) and Calpha1 isoforms of the catalytic subunit of cyclic 3',5'-adenosine monophosphate-dependent protein kinase testicular cells, Jovenal T. San Agustin and George B. Witman (Biology of reproduction)

Link

Transport and arrangement of the outer-dynein-arm docking complex in the flagella of Chlamydomonas mutants that lack outer dynein arms, Ken-ichi Wakabayashi, Saeko Takada, George B. Witman, and Ritsu Kamiya (Cell motility and the cytoskeleton)

Submissions from 2000

PDF

Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella, Gregory J. Pazour, Bethany L. Dickert, Yvonne Vucica, E. Scott Seeley, Joel L. Rosenbaum, George B. Witman, and Douglas G. Cole (The Journal of cell biology)

PDF

The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells, Jovenal T. San Agustin, Curtis G. Wilkerson, and George B. Witman (Molecular biology of the cell)

Submissions from 1999

Link

An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning, Cynthia J. Horst, Douglas J. Fishkind, Gregory J. Pazour, and George B. Witman (Cell motility and the cytoskeleton)

Link

Rotation of the central pair microtubules in eukaryotic flagella, Charlotte K. Omoto, Ian R. Gibbons, Ritsu Kamiya, Chikako Shingyoji, Keiichi Takahashi, and George B. Witman (Molecular biology of the cell)

PDF

The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly, Gregory J. Pazour, Bethany L. Dickert, and George B. Witman (The Journal of cell biology)

PDF

LC2, the chlamydomonas homologue of the t complex-encoded protein Tctex2, is essential for outer dynein arm assembly, Gregory J. Pazour, Anthony Koutoulis, Sharon E. Benashski, Bethany L. Dickert, Hong Sheng, Ramila S. Patel-King, Stephen M. King, and George B. Witman (Molecular biology of the cell)

Submissions from 1998

Link

A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT), Gregory J. Pazour, Curtis G. Wilkerson, and George B. Witman (The Journal of cell biology)

Link

The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence, Jovenal T. San Agustin, John D. Leszyk, Lydia M. Nuwaysir, and George B. Witman (The Journal of biological chemistry)

Submissions from 1997

Link

The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex, Anthony Koutoulis, Gregory J. Pazour, Curtis G. Wilkerson, Kazuo Inaba, Hong Sheng, Saeko Takada, and George B. Witman (The Journal of cell biology)

Link

Functional interaction between Chlamydomonas outer arm dynein subunits: the gamma subunit suppresses the ATPase activity of the alpha beta dimer, Ken-ichi Nakamura, Curtis G. Wilkerson, and George B. Witman (Cell motility and the cytoskeleton)

Submissions from 1995

Link

Reactivation of Chlamydomonas cell models, Cynthia J. Horst and George B. Witman (Methods in cell biology)

Link

The 78,000-M(r) intermediate chain of Chlamydomonas outer arm dynein is a microtubule-binding protein, Stephen M. King, Ramila S. Patel-King, Curtis G. Wilkerson, and George B. Witman (The Journal of cell biology)

Link

Assay of Chlamydomonas phototaxis, Anthony G. Moss, Gregory J. Pazour, and George B. Witman (Methods in cell biology)

Link

Interspecies conservation of outer arm dynein intermediate chain sequences defines two intermediate chain subclasses, Kazuo Ogawa, Ritsu Kamiya, Curtis G. Wilkerson, and George B. Witman (Molecular biology of the cell)

Link

Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii, Gregory J. Pazour, Oleg A. Sineshchekov, and George B. Witman (The Journal of cell biology)

Link

Detection of flagellar protein kinases on polyvinylidene difluoride membranes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Jovenal T. San Agustin and George B. Witman (Methods in cell biology)

Link

Isolation of ram sperm flagella, Jovenal T. San Agustin and George B. Witman (Methods in cell biology)

Link

Preparation and reactivation of demembranated, cytosol-free ram spermatozoa, Jovenal T. San Agustin and George B. Witman (Methods in cell biology)

Link

The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly, Curtis G. Wilkerson, Stephen M. King, Anthony Koutoulis, Gregory J. Pazour, and George B. Witman (The Journal of cell biology)

Submissions from 1994

Link

Multiple sites of phosphorylation within the alpha heavy chain of Chlamydomonas outer arm dynein, Stephen M. King and George B. Witman (The Journal of biological chemistry)

Link

Novel touch-induced, Ca(2+)-dependent phobic response in a flagellate green alga, Georg Kreimer and George B. Witman (Cell motility and the cytoskeleton)

Link

Role of cAMP in the reactivation of demembranated ram spermatozoa, Jovenal T. San Agustin and George B. Witman (Cell motility and the cytoskeleton)

Link

Molecular analysis of the gamma heavy chain of Chlamydomonas flagellar outer-arm dynein, Curtis G. Wilkerson, Stephen M. King, and George B. Witman (Journal of cell science)

Link

Electron micrographic studies of transport of oligodeoxynucleotides across eukaryotic cell membranes, Paul Zamecnik, John Aghajanian, Mary Zamecnik, John Goodchild, and George B. Witman (Proceedings of the National Academy of Sciences of the United States of America)

Submissions from 1993

Link

ptx1, a nonphototactic mutant of Chlamydomonas, lacks control of flagellar dominance, Cynthia J. Horst and George B. Witman (The Journal of cell biology)

Link

A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain, Hitoshi Sakakibara, Saeko Takada, Stephen M. King, George B. Witman, and Ritsu Kamiya (The Journal of cell biology)

Link

Reactivation of demembranated, cytosol-free ram spermatozoa, Jovenal T. San Agustin and George B. Witman (Cell motility and the cytoskeleton)

Link

Chlamydomonas phototaxis, George B. Witman (Trends in cell biology)

Submissions from 1992

Link

The motile beta/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond, Anthony G. Moss, Jean-Luc Gatti, and George B. Witman (The Journal of cell biology)

Link

The alpha subunit of sea urchin sperm outer arm dynein mediates structural and rigor binding to microtubules, Anthony G. Moss, Winfield S. Sale, Laura A. Fox, and George B. Witman (The Journal of cell biology)

Link

Axonemal dyneins, George B. Witman (Current opinion in cell biology)

Submissions from 1991

Link

Demembranation and reactivation of mammalian spermatozoa from golden hamster and ram, Sumio Ishijima and George B. Witman (Methods in enzymology)

Link

The Mr 78,000 intermediate chain of Chlamydomonas outer arm dynein interacts with alpha-tubulin in situ, Stephen M. King, Curtis G. Wilkerson, and George B. Witman (The Journal of biological chemistry)

Link

Purification and characterization of Salmo gairdneri outer arm dynein, Anthony G. Moss, Jean-Luc Gatti, Stephen M. King, and George B. Witman (Methods in enzymology)

Submissions from 1990

Link

Outer-arm dynein from trout spermatozoa: substructural organization, Stephen M. King, Jean-Luc Gatti, Anthony G. Moss, and George B. Witman (Cell motility and the cytoskeleton)

Link

Localization of an intermediate chain of outer arm dynein by immunoelectron microscopy, Stephen M. King and George B. Witman (The Journal of biological chemistry)

Submissions from 1989

Link

Outer arm dynein from trout spermatozoa. Purification, polypeptide composition, and enzymatic properties, Jean-Luc Gatti, Stephen M. King, Anthony G. Moss, and George B. Witman (The Journal of biological chemistry)

Link

Structure of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Nucleotide binding sites, Stephen M. King, Boyd E. Haley, and George B. Witman (The Journal of biological chemistry)

Submissions from 1988

Link

Structure of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Location of epitopes and protease-sensitive sites, Stephen M. King and George B. Witman (The Journal of biological chemistry)

Link

Structure of the gamma heavy chain of the outer arm dynein from Chlamydomonas flagella, Stephen M. King and George B. Witman (The Journal of cell biology)

Submissions from 1987

Link

Flagellar movement of intact and demembranated, reactivated ram spermatozoa, Sumio Ishijima and George B. Witman (Cell motility and the cytoskeleton)

Link

Structure of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Masses of chains and sites of ultraviolet-induced vanadate-dependent cleavage, Stephen M. King and George B. Witman (The Journal of biological chemistry)

Link

A two-step procedure for efficient electrotransfer of both high-molecular-weight (greater than 400,000) and low-molecular-weight (less than 20,000) proteins, Tim Otter, Stephen M. King, and George B. Witman (Analytical biochemistry)

Link

Isolated flagellar outer arm dynein translocates brain microtubules in vitro, Bryce Mark Paschal, Stephen M. King, Anthony G. Moss, Christine A. Collins, Richard B. Vallee, and George B. Witman (Nature)

Submissions from 1986

Link

Purification and characterization of Chlamydomonas flagellar dyneins, Stephen M. King, Tim Otter, and George B. Witman (Methods in enzymology)

Link

Isolation of Chlamydomonas flagella and flagellar axonemes, George B. Witman (Methods in enzymology)

Submissions from 1985

Link

Basal bodies and associated structures are not required for normal flagellar motion or phototaxis in the green alga Chlorogonium elongatum, Harold J. Hoops and George B. Witman (The Journal of cell biology)

Link

Characterization of monoclonal antibodies against Chlamydomonas flagellar dyneins by high-resolution protein blotting, Stephen M. King, Tim Otter, and George B. Witman (Proceedings of the National Academy of Sciences of the United States of America)

Link

Labeling of Chlamydomonas 18 S dynein polypeptides by 8-azidoadenosine 5'-triphosphate, a photoaffinity analog of ATP, K. Kevin Pfister, Boyd E. Haley, and George B. Witman (The Journal of biological chemistry)

Submissions from 1984

Link

Flagellar waveform and rotational orientation in a Chlamydomonas mutant lacking normal striated fibers, Harold J. Hoops, Robin L. Wright, Jonathan W. Jarvik, and George B. Witman (The Journal of cell biology)

Link

Submicromolar levels of calcium control the balance of beating between the two flagella in demembranated models of Chlamydomonas, Ritsu Kamiya and George B. Witman (The Journal of cell biology)

Link

The photoaffinity probe 8-azidoadenosine 5'-triphosphate selectively labels the heavy chain of Chlamydomonas 12 S dynein, K. Kevin Pfister, Boyd E. Haley, and George B. Witman (The Journal of biological chemistry)

Link

Subfractionation of Chlamydomonas 18 S dynein into two unique subunits containing ATPase activity, K. Kevin Pfister and George B. Witman (The Journal of biological chemistry)

Submissions from 1983

Link

Outer doublet heterogeneity reveals structural polarity related to beat direction in Chlamydomonas flagella, Harold J. Hoops and George B. Witman (The Journal of cell biology)

Submissions from 1982

Link

Purification and polypeptide composition of dynein ATPases from Chlamydomonas flagella, K. Kevin Pfister, Rose B. Fay, and George B. Witman (Cell motility)

Link

Synthesis, transport, and utilization of specific flagellar proteins during flagellar regeneration in Chlamydomonas, Stephen P. Remillard and George B. Witman (The Journal of cell biology)

Link

Role of calmodulin in the flagellar axoneme: effect of phenothiazines on reactivated axonemes of Chlamydomonas, George B. Witman and N. Minervini (Progress in clinical and biological research)

Submissions from 1981

Link

Functionally significant central-pair rotation in a primitive eukaryotic flagellum, Charlotte K. Omoto and George B. Witman (Nature)