UMMS Affiliation

Department of Pediatrics; Department of Medicine; Center for Infectious Disease and Vaccine Research; Graduate School of Biomedical Sciences, Clinical and Population Health Research Program

Date

8-1-2011

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences | Women's Studies

Abstract

Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which are predominantly serotype-cross-reactive regardless of DENV infection history. These data confirm marked T cell activation and serotype-cross-reactivity during the febrile phase of dengue; however, A11-NS3(133)-specific responses did not correlate with prior antigenic exposure or current disease severity.

Rights and Permissions

Citation: Sci Rep. 2011;1:51. Epub 2011 Aug 1. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

22355570

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