Title
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes
UMMS Affiliation
Department of Cell Biology
Date
4-10-1992
Document Type
Article
Subjects
Actins; Alkaloids; Animals; Calcium; Cell Membrane; Dictyostelium; Diglycerides; Kinetics; Macromolecular Substances; *Naphthalenes; Polycyclic Compounds; Protein Kinase C; Staurosporine; Tetradecanoylphorbol Acetate; Time Factors
Disciplines
Cell Biology | Life Sciences | Medicine and Health Sciences
Abstract
Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence of highly purified plasma membranes from the cellular slime mold Dictyostelium discoideum. The increased rate of actin polymerization apparently resulted from de novo formation of actin nucleation sites rather than uncapping of existing filament ends, because the membranes lacked detectable endogenous actin. The increased actin nucleation was mediated by a peripheral membrane component other than protein kinase C, the classical target of diacylglycerol action. These results indicate that diacylglycerols increase actin nucleation at plasma membranes and suggest a mechanism whereby signal transduction pathways may control cytoskeletal assembly.
Rights and Permissions
Citation: Science. 1992 Apr 10;256(5054):245-7.
Related Resources
PubMed ID
1373523
