Title

Detection and characterization of RNA tumor virus-specific DNA in cells

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Date

1-1-1975

Document Type

Article

Subjects

Animals; Avian Sarcoma Viruses; Cell Line; DNA, Single-Stranded; DNA, Viral; Humans; Mammary Tumor Virus, Mouse; Mice; Nucleic Acid Hybridization; RNA Viruses; RNA-Directed DNA Polymerase; Sarcoma, Experimental

Disciplines

Cancer Biology | Microbiology | Molecular Genetics

Abstract

RNA tumor virus-specific DNA in cells can be detected by its capacity to 1) alter the reassociation kinetics of labeled double-stranded product of viral RNA-directed DNA polymerase; 2) anneal single-stranded DNA (cDNA) synthesized by viral polymerase; or 3) hybridize labeled viral 70S (genomic) RNA. Duplexes formed with these procedures can be analyzed for fidelity of base pairing, and the integration of viral DNA into the host genome can be established with a simple but stringent technique. We illustrate this methodology as applied to detection of Rous sarcoma virus (RSV)-specific DNA in XC cells and of mouse mammary tumor virus (MMTV)-specific DNA in murine and human tissues.

Rights and Permissions

Citation: Bibl Haematol. 1975;(40):451-61.

Related Resources

Link to article in PubMed

PubMed ID

51630

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