Title
Detection and characterization of RNA tumor virus-specific DNA in cells
UMMS Affiliation
Department of Molecular Genetics and Microbiology
Date
1-1-1975
Document Type
Article
Subjects
Animals; Avian Sarcoma Viruses; Cell Line; DNA, Single-Stranded; DNA, Viral; Humans; Mammary Tumor Virus, Mouse; Mice; Nucleic Acid Hybridization; RNA Viruses; RNA-Directed DNA Polymerase; Sarcoma, Experimental
Disciplines
Cancer Biology | Microbiology | Molecular genetics
Abstract
RNA tumor virus-specific DNA in cells can be detected by its capacity to 1) alter the reassociation kinetics of labeled double-stranded product of viral RNA-directed DNA polymerase; 2) anneal single-stranded DNA (cDNA) synthesized by viral polymerase; or 3) hybridize labeled viral 70S (genomic) RNA. Duplexes formed with these procedures can be analyzed for fidelity of base pairing, and the integration of viral DNA into the host genome can be established with a simple but stringent technique. We illustrate this methodology as applied to detection of Rous sarcoma virus (RSV)-specific DNA in XC cells and of mouse mammary tumor virus (MMTV)-specific DNA in murine and human tissues.
Rights and Permissions
Citation: Bibl Haematol. 1975;(40):451-61.
Related Resources
PubMed ID
51630
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