Title

Predictive testing with the subrenal capsule assay

UMMS Affiliation

Department of Medicine, Division of Hematology/Oncology

Date

3-1984

Document Type

Article

Subjects

Altretamine; Animals; *Antineoplastic Agents; Cyclophosphamide; Dacarbazine; Drug Evaluation, Preclinical; Drug Resistance; Humans; Kidney; Mice; Necrosis; Neoplasm Transplantation; Neoplasms; Prospective Studies; Transplantation, Heterologous

Disciplines

Life Sciences | Medicine and Health Sciences | Women's Studies

Abstract

Measurement of drug activity as an oncolytic effect, use of the control only to monitor the quality of tissue implanted, and the rapid clearance of necrotic tissue from the subcapsular site, as significant factors incorporated into the design of the assay, have permitted use of a simple tumor size parameter for evaluating drug activity. The simplicity and economy of such a parameter, the predictability and reproducibility of the 6-day assay observed thus far, and evidence that the assay does measure a biological property of the tumor apart from host response, have warranted the continued use of the 6-day time frame and the normal immunocompetent CDF1 mouse as xenograft host. These studies have demonstrated the feasibility of using human tumor explants obtained from a variety of solid human malignancies in a straightforward, short term, in vivo predictive assay system. Preliminary correlations between in vivo (assay) tumor sensitivity and clinical response have given reasonable concurrence. This crucial point will require further study, with larger numbers of patients, under more rigid conditions. Final validation of this, and other, predictive assays will require a prospective, randomized study in large numbers of patients. Our present prospective study is being continued, therefore, with expansion to a multi-institutional design over a broader geographic area.

Rights and Permissions

Citation: Cancer Treat Rev. 1984 Mar;11 Suppl A:113-24.

Related Resources

Link to article in PubMed

PubMed ID

6428738