Kinetic mechanism of protein arginine methyltransferase 6 (PRMT6).
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Amino Acid Sequence; Biocatalysis; Drug Discovery; Enzyme Inhibitors; Humans; Kinetics; Nuclear Proteins; Peptides; Protein-Arginine N-Methyltransferases
Biochemistry | Enzymes and Coenzymes | Medicinal-Pharmaceutical Chemistry | Therapeutics
The protein arginine methyltransferases (PRMTs) are a family of enzymes that catalyze the mono- and dimethylation of arginine residues in a variety of proteins. Although these enzymes play important roles in a variety of cellular processes, aberrant PRMT activity is associated with several disease states, including heart disease and cancer. In an effort to guide the development of inhibitors targeting individual PRMTs, we initiated studies to characterize the molecular mechanisms of PRMT catalysis. Herein, we report studies on the kinetic mechanism of PRMT6. Initial velocity, product inhibition, and dead-end analog inhibition studies with the AcH4-21 and R1 peptides, as well as their monomethylated versions, indicate, in contrast to a previous report, that PRMT6 utilizes a rapid equilibrium random mechanism with dead-end EAP and EBQ complexes.
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Citation: J Biol Chem. 2012 Feb 17;287(8):6062-71. doi: 10.1074/jbc.M111.333609. Epub 2012 Jan 3. Link to article on publisher's site
Obianyo, Obiamaka and Thompson, Paul R., "Kinetic mechanism of protein arginine methyltransferase 6 (PRMT6)." (2012). Thompson Lab Publications. 39.