Title

Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

5-20-2015

Document Type

Article

Disciplines

Biochemistry | Cancer Biology | Enzymes and Coenzymes | Laboratory and Basic Science Research | Medicinal-Pharmaceutical Chemistry | Therapeutics

Abstract

Here, we document the use of the soft agar colony formation assay to test the effects of a peptidylarginine deiminase (PADI) enzyme inhibitor, BB-Cl-amidine, on breast cancer tumorigenicity in vitro.

Rights and Permissions

Citation: Horibata, S., Vo, T. V., Subramanian, V., Thompson, P. R., Coonrod, S. A. Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells. J. Vis. Exp. (99), e52727, doi:10.3791/52727 (2015).

Related Resources

Link to article in PubMed