The Hitchhiker's guide to Hi-C analysis: Practical guidelines
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Bioinformatics | Computational Biology | Genetics and Genomics | Systems Biology
Over the last decade, development and application of a set of molecular genomic approaches based on the chromosome conformation capture method (3C), combined with increasingly powerful imaging approaches, have enabled high resolution and genome-wide analysis of the spatial organization of chromosomes. The aim of this paper is to provide guidelines for analyzing and interpreting data obtained with genome-wide 3C methods such as Hi-C and 3C-seq that rely on deep sequencing to detect and quantify pairwise chromatin interactions.
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Citation: Methods. 2014 Nov 6. pii: S1046-2023(14)00358-2. doi: 10.1016/j.ymeth.2014.10.031. [Epub ahead of print]. Link to article on publisher's site
Bioinformatics, Chromatin structure, Chromosome conformation capture, Deep sequencing, Hi-C
Methods (San Diego, Calif.)
Lajoie, Bryan R.; Dekker, Job; and Kaplan, Noam, "The Hitchhiker's guide to Hi-C analysis: Practical guidelines" (2014). Program in Systems Biology Publications and Presentations. 55.