The Hierarchy of the 3D Genome
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Genome; Chromosome Structures; Protein Conformation; Protein Folding; Gene Expression Regulation
Genetics and Genomics | Molecular Biology | Systems Biology
Mammalian genomes encode genetic information in their linear sequence, but appropriate expression of their genes requires chromosomes to fold into complex three-dimensional structures. Transcriptional control involves the establishment of physical connections among genes and regulatory elements, both along and between chromosomes. Recent technological innovations in probing the folding of chromosomes are providing new insights into the spatial organization of genomes and its role in gene regulation. It is emerging that folding of large complex chromosomes involves a hierarchy of structures, from chromatin loops that connect genes and enhancers to larger chromosomal domains and nuclear compartments. The larger these structures are along this hierarchy, the more stable they are within cells, while becoming more stochastic between cells. Here, we review the experimental and theoretical data on this hierarchy of structures and propose a key role for the recently discovered topologically associating domains.
Gibcus, Johan H. and Dekker, Job, "The Hierarchy of the 3D Genome" (2013). Program in Systems Biology Publications and Presentations. 20.