Title
Analysis of long-range chromatin interactions using Chromosome Conformation Capture
UMMS Affiliation
Program in Systems Biology; Program in Gene Function and Expression; Department of Biochemistry and Molecular Pharmacology
Date
11-2012
Document Type
Article
Medical Subject Headings
Chromatin; Chromosomes; Nucleic Acid Conformation
Disciplines
Genetics and Genomics | Systems Biology
Abstract
Chromosome Conformation Capture, or 3C, is a pioneering method for investigating the three-dimensional structure of chromatin. 3C is used to analyze long-range looping interactions between any pair of selected genomic loci. Most 3C studies focus on defined genomic regions of interest that can be up to several hundred Kb in size. The method has become widely adopted and has been modified to increase throughput to allow unbiased genome-wide analysis. These large-scale adaptations are presented in other articles in this issue of Methods. Here we describe the 3C procedure in detail, including the appropriate use of the technology, the experimental set-up, an optimized protocol and troubleshooting guide, and considerations for data analysis. The protocol described here contains previously unpublished improvements, which save time and reduce labor. We pay special attention to primer design, appropriate controls and data analysis. We include notes and discussion based on our extensive experience to help researchers understand the principles of 3C-based techniques and to avoid common pitfalls and mistakes. This paper represents a complete resource and detailed guide for anyone who desires to perform 3C.
Rights and Permissions
Citation: Methods. 2012 Nov;58(3):192-203. doi: 10.1016/j.ymeth.2012.07.022. Epub 2012 Aug 15. Link to article on publisher's site