UMass Chan Affiliations
UMass Metabolic NetworkProgram in Molecular Medicine
Program in Systems Biology
Document Type
Journal ArticlePublication Date
2016-12-01
Metadata
Show full item recordAbstract
In this protocol, we present a qualitative assay for monitoring the level of expression of beta-galactosidase, an enzyme encoded by the LacZ gene, in yeast. This is useful both for determining autoactivity of LacZ expression in yeast DNA "bait" strains and for assessing LacZ reporter gene activation mediated by a transcription factor "prey" interaction with a DNA bait of interest in yeast one-hybrid (Y1H) assays. In this colorimetric assay, yeast are lysed in liquid nitrogen and then assayed for beta-galactosidase expression using the colorless compound X-gal, which turns blue in the presence of this enzyme.Source
Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot088963. doi: 10.1101/pdb.prot088963 Link to article on publisher's siteDOI
10.1101/pdb.prot088963Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49830PubMed ID
27934685Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot088963