Title

Zymolyase-Treatment and Polymerase Chain Reaction Amplification from Genomic and Plasmid Templates from Yeast

UMMS Affiliation

Program in Systems Biology; Program in Molecular Medicine; UMass Metabolic Network

Date

12-1-2016

Document Type

Article

Disciplines

Genetics and Genomics | Laboratory and Basic Science Research | Molecular Biology | Systems Biology

Abstract

Here, we present a protocol for amplifying DNA fragments from the genome of, or plasmids transformed into, yeast strains that require the use of the lytic enzyme zymolyase to break open the yeast cells by digesting the cell wall. Yeast strains requiring such treatment include YM4271 and Y1HaS2, whereas other yeast strains (e.g., MaV103) might not require treatment with Zymolyase.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot088971. doi: 10.1101/pdb.prot088971. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Cold Spring Harbor protocols

PubMed ID

27934686