Zymolyase-Treatment and Polymerase Chain Reaction Amplification from Genomic and Plasmid Templates from Yeast
Program in Systems Biology; Program in Molecular Medicine; UMass Metabolic Network
Genetics and Genomics | Laboratory and Basic Science Research | Molecular Biology | Systems Biology
Here, we present a protocol for amplifying DNA fragments from the genome of, or plasmids transformed into, yeast strains that require the use of the lytic enzyme zymolyase to break open the yeast cells by digesting the cell wall. Yeast strains requiring such treatment include YM4271 and Y1HaS2, whereas other yeast strains (e.g., MaV103) might not require treatment with Zymolyase.
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Citation: Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot088971. doi: 10.1101/pdb.prot088971. Link to article on publisher's site
Cold Spring Harbor protocols
Fuxman Bass, Juan; Reece-Hoyes, John S.; and Walhout, Albertha J. M., "Zymolyase-Treatment and Polymerase Chain Reaction Amplification from Genomic and Plasmid Templates from Yeast" (2016). Program in Systems Biology Publications and Presentations. 104.