Title

Proximal promoter binding protein contributes to developmental, tissue-restricted expression of the rat osteocalcin gene

UMMS Affiliation

Department of Cell Biology

Date

1-1-1995

Document Type

Article

Medical Subject Headings

Animals; Base Sequence; Cell Differentiation; Cell Extracts; DNA; Gene Expression Regulation, Developmental; Humans; Molecular Sequence Data; Organ Specificity; Osteoblasts; Osteocalcin; Promoter Regions, Genetic; Rats

Disciplines

Cell Biology

Abstract

Osteocalcin is a 6 kD tissue-specific calcium binding protein associated with the bone extracellular matrix. The osteocalcin gene is developmentally expressed in postoproliferative rat osteoblasts with regulation at least in part at the transcriptional level. Multiple, basal promoter and enhancer elements which control transcriptional activity in response to physiological mediators, including steroid hormones, have been identified in the modularly organized osteocalcin gene promoter. The osteocalcin box (OC box) is a highly conserved basal regulatory element residing between nucleotides -99 and -76 of the proximal promoter. We recently established by in vivo competition analysis that protein interactions at the CCAAT motif, which is the central core of the rat OC box, are required for support of basal transcription [Heinrichs et al. J Cell Biochem 53:240-250, 1993]. In this study, by the combined utilization of electrophoretic mobility shift analysis, UV cross linking, and DNA affinity chromatography, we have identified a protein that binds to the rat OC box. Results are presented that support involvement of the OC box-binding protein in regulating selective expression of the osteocalcin gene during differentiation of the rat osteoblast phenotype and suggest that this protein is tissue restricted.

Rights and Permissions

Citation: J Cell Biochem. 1995 Jan;57(1):90-100. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

7721961