Title

Histone H4 proximal promoter mediates a complex transcriptional response during differentiation of 3T3L1 adipocytes

UMMS Affiliation

Department of Cell Biology

Date

5-1-1995

Document Type

Article

Medical Subject Headings

3T3 Cells; Adipocytes; Animals; Cell Differentiation; Chloramphenicol O-Acetyltransferase; Cloning, Molecular; Gene Expression; Gene Expression Regulation; Histones; Humans; Mice; *Promoter Regions, Genetic; *Transcription, Genetic

Disciplines

Cell Biology

Abstract

We have investigated the promoter element(s) required by the cell cycle regulated FO108 human histone H4 gene for control of gene expression during adipocyte proliferation and differentiation. Stable 3T3L1 cell lines were established that express fusion genes in which the histone H4 promoter is joined to chloramphenicol acetyltransferase (cat) as a reporter gene. Expression of the H4CAT fusion genes was monitored in proliferating and confluent 3T3L1 preadipocytes and in differentiating 3T3L1 adipocytes. The results indicate that the H4 cell cycle element (CCE), which mediates S phase-specific stimulation of H4 gene transcription, is not required for transcriptional regulation during differentiation. Instead, a minimal H4 promoter (nucleotides -46 to -11) is sufficient to mediate the complex transcriptional response of H4 gene expression observed during the process of adipocyte differentiation of 3T3L1 cells. In addition, the data suggest that down-regulation of histone gene expression during cellular differentiation may be mediated by passive inactivation of the promoter due to loss of positive regulatory factor(s).

Rights and Permissions

Citation: J Cell Physiol. 1995 May;163(2):312-20. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

7706376