Title

Subnuclear localization and intranuclear trafficking of transcription factors

UMMS Affiliation

Department of Cell Biology

Date

8-10-2010

Document Type

Article

Medical Subject Headings

Intranuclear Space

Disciplines

Cell Biology

Abstract

Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Rights and Permissions

Citation: Methods Mol Biol. 2010;647:77-93. Link to article on publisher's site

Related Resources

Link to Article in PubMed