Subnuclear localization and intranuclear trafficking of transcription factors

UMMS Affiliation

Department of Cell Biology



Document Type


Medical Subject Headings

Intranuclear Space


Cell Biology


Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

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Citation: Methods Mol Biol. 2010;647:77-93. Link to article on publisher's site

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Link to Article in PubMed