Heparan Sulfate Enhances the Self-Renewal and Therapeutic Potential of Mesenchymal Stem Cells from Human Adult Bone Marrow
Department of Cell Biology
Medical Subject Headings
Mesenchymal Stem Cells; Heparitin Sulfate
Insufficient cell number hampers therapies utilizing adult human mesenchymal stem cells (hMSCs) and current ex vivo expansion strategies lead to a loss of multipotentiality. Here we show that supplementation with an embryonic form of heparan sulfate (HS-2) can both increase the initial recovery of hMSCs from bone marrow aspirates and increase their ex vivo expansion by up to 13-fold. HS-2 acts to amplify a subpopulation of hMSCs harboring longer telomeres and increased expression of the MSC surface marker stromal precursor antigen-1. Gene expression profiling revealed that hMSCs cultured in HS-2 possess a distinct signature that reflects their enhanced multipotentiality and improved bone-forming ability when transplanted into critical-sized bone defects. Thus, HS-2 offers a novel means for decreasing the expansion time necessary for obtaining therapeutic numbers of multipotent hMSCs without the addition of exogenous growth factors that compromise stem cell fate.
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Citation: Stem Cells Dev. 2012 Jan 18. Link to article on publisher's site
Helledie, Torben; Dombrowski, Christian; Rai, Bina; Lim, Zophia X.H.; Hin, Ian Lee Hock; Rider, David A.; Stein, Gary S.; Hong, Wanjin; Van Wijnen, Andre J.; Hui, James H.; Nurcombe, Victor; and Cool, Simon M., "Heparan Sulfate Enhances the Self-Renewal and Therapeutic Potential of Mesenchymal Stem Cells from Human Adult Bone Marrow" (2012). Stein, Stein, Lian, vanWijnen Lab Publications. 247.