Title

Evidence for fidelity of chromatin reconstitution

UMMS Affiliation

Department of Cell Biology

Date

5-6-1975

Document Type

Article

Medical Subject Headings

Animals; Cell Nucleus; Chromatin; Chromosomes; Circular Dichroism; DNA; DNA, Neoplasm; DNA-Directed RNA Polymerases; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Hela Cells; Histones; Humans; Liver; Nucleic Acid Conformation; Rats; Transcription, Genetic

Disciplines

Cell Biology

Abstract

Several lines of evidence are presented which support the contention that chromatin may be dissociated, fractionated, and reconstituted without altering the compositional, structural, or transcriptional integrity of the genome. The similar compositions of native and reconstituted chromatins are suggested by the absence of significant differences in their protein/DNA ratios and in the polyacrylamide gel electrophoretic profiles of their histones and nonhistone chromosomal proteins. Criteria for fidelity of genome structure in reconstituted chromatin include binding of reporter molecules with specificity for the minor groove of DNA, binding of histones, number of sites available for addition of nucleotides, and circular dichroism spectra. When the transcriptional activities of native and reconstituted chromatins were compared under conditions where reinitiation is prohibited, significant changes were not observed. Taken together, the present results strongly suggest, but do not conclusively establish, fidelity of chromatin reconstitution.

Rights and Permissions

Citation: Biochemistry. 1975 May 6;14(9):1859-66.

Related Resources

Link to Article in PubMed

PubMed ID

1092344