Title

Synthesis of nuclear proteins during DNA repair synthesis in human diploid fibroblasts damaged with ultraviolet radiation of N-acetoxy-2-acetylaminofluroene

UMMS Affiliation

Department of Cell Biology

Date

5-1-1976

Document Type

Article

Medical Subject Headings

Acetoxyacetylaminofluorene; Cell Line; Cell Nucleus; *DNA Repair; Histones; Hydroxyurea; Nucleoproteins; Radiation Effects; Ultraviolet Rays

Disciplines

Cell Biology

Abstract

We have examined the accumulation of newly synthesized nuclear proteins into nuclei during DNA repair synthesis in confluent WI-38 human diploid fibroblasts damaged with ultraviolet radiation or N-acetoxy-2-acetylaminofluroene. In contrast to a marked stimulation of DNA repair synthesis, stimulation of amino acid incorporation into histone polypeptides or into the various molecular weight classes of nonhistone nuclear proteins was not observed. These results suggest that detectable stimulation of newly synthesized nuclear protein incorporation into nuclei does not accompany DNA repair synthesis induced by ultraviolet radiation or a direct acting chemical carcinogen. At least for the special case of repair, DNA synthesis may be uncoupled from histone synthesis.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 1976 May;73(5):1466-70.

Related Resources

Link to Article in PubMed

PubMed ID

1064020