Title

A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program

UMMS Affiliation

Department of Cell Biology

Date

10-29-2010

Document Type

Article

Medical Subject Headings

Cell Differentiation; Core Binding Factor Alpha 1 Subunit; Gene Regulatory Networks; Matrix Attachment Region Binding Proteins; MicroRNAs; Multigene Family; Osteoblasts; Transcription Factors

Disciplines

Cell Biology

Abstract

Induced osteogenesis includes a program of microRNAs (miRs) to repress the translation of genes that act as inhibitors of bone formation. How expression of bone-related miRs is regulated remains a compelling question. Here we report that Runx2, a transcription factor essential for osteoblastogenesis, negatively regulates expression of the miR cluster 23a approximately 27a approximately 24-2. Overexpression, reporter, and chromatin immunoprecipitation assays established the presence of a functional Runx binding element that represses expression of these miRs. Consistent with this finding, exogenous expression of each of the miRs suppressed osteoblast differentiation, whereas antagomirs increased bone marker expression. The biological significance of Runx2 repression of this miR cluster is that each miR directly targets the 3' UTR of SATB2, which is known to synergize with Runx2 to facilitate bone formation. The findings suggest Runx2-negative regulation of multiple miRs by a feed-forward mechanism to cause derepression of SATB2 to promote differentiation. We find also that miR-23a represses Runx2 in the terminally differentiated osteocyte, representing a feedback mechanism to attenuate osteoblast maturation. We provide direct evidence for an interdependent relationship among transcriptional inhibition of the miR cluster by Runx2, translational repression of Runx2 and of SATB2 by the cluster miRs during progression of osteoblast differentiation. Furthermore, miR cluster gain of function (i.e., inhibition of osteogenesis) is rescued by the exogenous expression of SATB2. Taken together, we have established a regulatory network with a central role for the miR cluster 23a approximately 27a approximately 24-2 in both progression and maintenance of the osteocyte phenotype.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19879-84. Epub 2010 Oct 27. Link to article on publisher's site

Related Resources

Link to Article in PubMed