Multiple forms of H4 histone mRNA in human cells
Department of Cell Biology
Medical Subject Headings
Cell Cycle; Hela Cells; Histones; Humans; Molecular Weight; Nucleic Acid Conformation; Polyribosomes; RNA Caps; RNA, Messenger
Two species of H4 histone mRNA were isolated from the polysomes of S phase HeLa S3 cells. Electrophoresis under denaturing and nondenaturing conditions indicates that the two H4 mRNAs differ in size. Both mRNAs translate H4 histones in vitro, lack poly(A) at their 3' termini, and are capped at their 5' termini. Polyacrylamide gel electrophoresis and tryptic peptide analysis suggest that the polypeptides synthesized by the two mRNAs are indistinguishable.
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Citation: Proc Natl Acad Sci U S A. 1980 Apr;77(4):1942-6.
Lichtler, A. C.; Detke, Siegfried; Phillips, Ian R.; Stein, Gary S.; and Stein, Janet L., "Multiple forms of H4 histone mRNA in human cells" (1980). Stein, Stein, Lian, vanWijnen Lab Publications. 173.