Title

Multiple forms of H4 histone mRNA in human cells

UMMS Affiliation

Department of Cell Biology

Date

4-1-1980

Document Type

Article

Medical Subject Headings

Cell Cycle; Hela Cells; Histones; Humans; Molecular Weight; Nucleic Acid Conformation; Polyribosomes; RNA Caps; RNA, Messenger

Disciplines

Cell Biology

Abstract

Two species of H4 histone mRNA were isolated from the polysomes of S phase HeLa S3 cells. Electrophoresis under denaturing and nondenaturing conditions indicates that the two H4 mRNAs differ in size. Both mRNAs translate H4 histones in vitro, lack poly(A) at their 3' termini, and are capped at their 5' termini. Polyacrylamide gel electrophoresis and tryptic peptide analysis suggest that the polypeptides synthesized by the two mRNAs are indistinguishable.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 1980 Apr;77(4):1942-6.

Related Resources

Link to Article in PubMed

PubMed ID

6929528