Title

Nuclear antigens in the HeLa cell cycle

UMMS Affiliation

Department of Cell Biology

Date

7-1-1985

Document Type

Article

Medical Subject Headings

Antigens; Antigens, Nuclear; Cell Cycle; Hela Cells; Humans; Interphase; Metaphase; Nucleoproteins

Disciplines

Cell Biology

Abstract

Antisera to 0.35 M NaCl extracts and residues of S phase HeLa nuclei were reacted with electrophoretically separated proteins from the nuclei or nuclear material of HeLa cells synchronized in G1, S, G2 or M phases of the cell cycle. Quantitative evaluation of the peroxidase-antiperoxidase stained nitrocellulose transfers (Western blots) revealed significant changes in the quantities of nuclear non-histone proteins during the cell cycle. Immunochemical staining of electrophoretically separated nuclear antigens permits their selective detection in minute quantities and in the presence of many additional proteins.

Rights and Permissions

Citation: Mol Cell Biochem. 1985 Jul;67(2):101-10.

Related Resources

Link to Article in PubMed

PubMed ID

4047024