Title

Single-copy flanking sequences in human histone gene clusters map to chromosomes 1 and 6

UMMS Affiliation

Department of Cell Biology

Date

6-1-1991

Document Type

Article

Medical Subject Headings

*Chromosomes, Human, Pair 1; *Chromosomes, Human, Pair 6; Histones; Humans; Introns; *Multigene Family; Nucleic Acid Hybridization; Restriction Mapping

Disciplines

Cell Biology

Abstract

Two single-copy sequences flanking two different human histone gene clusters were used as probes to map these clusters by in situ hybridization. pFF435B, a unique sequence subclone derived from a lambda genomic clone (lambda HHG55) containing H2A, H2B, H3, and H4 genes, mapped to chromosome 1q21 (chi 2 = 120.99, P less than 0.001). pST519E, a single-copy sequence derived from a lambda genomic clone (lambda HHG17) containing only H3 and H4 genes, mapped to chromosome 6p21 (chi 2 = 112.62, P less than 0.001). These findings agree with previous assignments of human histone genes to chromosomes 1 and 6 and demonstrate that the single-copy flanking sequences in different human histone gene clusters are unique for different chromosomes.

Rights and Permissions

Citation: Genomics. 1991 Jun;10(2):486-8.

Related Resources

Link to Article in PubMed

PubMed ID

2071153