Single-copy flanking sequences in human histone gene clusters map to chromosomes 1 and 6
Department of Cell Biology
Medical Subject Headings
*Chromosomes, Human, Pair 1; *Chromosomes, Human, Pair 6; Histones; Humans; Introns; *Multigene Family; Nucleic Acid Hybridization; Restriction Mapping
Two single-copy sequences flanking two different human histone gene clusters were used as probes to map these clusters by in situ hybridization. pFF435B, a unique sequence subclone derived from a lambda genomic clone (lambda HHG55) containing H2A, H2B, H3, and H4 genes, mapped to chromosome 1q21 (chi 2 = 120.99, P less than 0.001). pST519E, a single-copy sequence derived from a lambda genomic clone (lambda HHG17) containing only H3 and H4 genes, mapped to chromosome 6p21 (chi 2 = 112.62, P less than 0.001). These findings agree with previous assignments of human histone genes to chromosomes 1 and 6 and demonstrate that the single-copy flanking sequences in different human histone gene clusters are unique for different chromosomes.
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Citation: Genomics. 1991 Jun;10(2):486-8.
Allen, Beverly S.; Stein, Janet L.; Stein, Gary S.; and Ostrer, Harry, "Single-copy flanking sequences in human histone gene clusters map to chromosomes 1 and 6" (1991). Stein, Stein, Lian, vanWijnen Lab Publications. 115.