Liquid ventilation with perflubron in the treatment of rats with pneumococcal pneumonia
Department of Physiology; Department of Emergency Medicine; Department of Anesthesiology; Department of Surgery
Medical Subject Headings
Animals; Anti-Bacterial Agents; Combined Modality Therapy; Fluorocarbons; Infusions, Parenteral; *Liquid Ventilation; Male; Pneumonia, Pneumococcal; Random Allocation; Rats; Rats, Wistar; Statistics, Nonparametric; Survival Analysis
Anesthesiology | Life Sciences | Medicine and Health Sciences | Respiratory Tract Diseases
OBJECTIVE: To determine the efficacy of liquid ventilation using a medical-grade perfluorocarbon (perflubron) combined with parenteral or intratracheal antibiotics in a rat model of pneumonia.
DESIGN: Prospective, laboratory investigation.
SETTING: Experimental laboratory in a university medical center.
SUBJECTS: Wistar rats (n = 112).
INTERVENTIONS: One day after intratracheal inoculation with Streptococcus pneumoniae, rats received one of five experimental treatments or no treatment (control): modified liquid ventilation (MLV), intramuscular ampicillin, MLV plus intramuscular ampicillin, MLV with intratracheal ampicillin, or MLV plus ampicillin PulmoSpheres.
MEASUREMENTS AND MAIN RESULTS: Animals receiving MLV plus intramuscular ampicillin, MLV with intratracheal ampicillin, or MLV plus ampicillin PulmoSpheres had significantly improved 10-day survival rates (85%, 72%, and 72%, respectively) compared with all other groups (0% to 25%).
CONCLUSIONS: MLV in combination with either intramuscular, intratracheal, or PulmoSpheres ampicillin improved survival as compared with MLV alone or the same dose of antibiotics delivered intramuscularly.
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Citation: Crit Care Med. 2002 Feb;30(2):393-5.
Dickson, Eric W.; Heard, Stephen O.; Tarara, Thomas E.; Weers, Jeffry G.; Brueggemann, Angela B.; and Doern, Gary V., "Liquid ventilation with perflubron in the treatment of rats with pneumococcal pneumonia" (2002). University of Massachusetts Medical School. Senior Scholars Program. Paper 78.