Link Between DNA Damage and Centriole Disengagement/Reduplication in Untransformed Human Cells
UMass Chan Affiliations
Department of Cell and Developmental BiologyDocument Type
Journal ArticlePublication Date
2014-02-14
Metadata
Show full item recordAbstract
The radiation and radiomimetic drugs used to treat human tumors damage DNA in both cancer cells and normal proliferating cells. Centrosome amplification after DNA damage is well established for transformed cell types but is sparsely reported and not fully understood in untransformed cells. We characterize centriole behavior after DNA damage in synchronized untransformed human cells. One hour treatment of S phase cells with the radiomimetic drug, Doxorubicin, prolongs G2 by at least 72 hours, though 14% of the cells eventually go through mitosis in that time. By 72 hours after DNA damage we observe a 52% incidence of centriole disengagement plus a 10% incidence of extra centrioles. We find that either APC/C or Plk activities can disengage centrioles after DNA damage, though they normally work in concert. All disengaged centrioles are associated with gamma-tubulin and maturation markers and thus, should in principle be capable of reduplicating and organizing spindle poles. The low incidence of reduplication of disengaged centrioles during G2 is due to the p53 dependent expression of p21 and the consequent loss of Cdk2 activity. We find that 26% of the cells going through mitosis after DNA damage contain disengaged or extra centrioles. This could produce genomic instability through transient or persistent spindle multipolarity. Thus, for cancer patients the use of DNA damaging therapies raises the chances of genomic instability and evolution of transformed characteristics in proliferating normal cell populations. J. Cell. Physiol. (c) 2014 Wiley Periodicals, Inc.Source
Douthwright S, Sluder G. J Cell Physiol. 2014 Feb 14. doi: 10.1002/jcp.24579. Link to article on publisher's siteDOI
10.1002/jcp.24579Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49024PubMed ID
24532022Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jcp.24579