Sluder Lab Publications

UMMS Affiliation

Department of Cell Biology

Date

7-25-2011

Document Type

Article

Medical Subject Headings

Animals; CHO Cells; Cell Proliferation; Cells, Cultured; Centrosome; Cricetinae; Cricetulus; HeLa Cells; Humans; Immunohistochemistry; Tumor Suppressor Protein p53

Disciplines

Cell Biology

Abstract

We tested whether cleavage failure as a transient event establishes an incidence of centrosome amplification in cell populations. Five rounds of approximately 30% cytochalasin-induced cleavage failure in untransformed human cell cultures did not establish centrosome amplification in the short or long terms. The progeny of binucleate cells progressively dropped out of the cell cycle and expressed p53/p21, and none divided a fourth time. We also tested whether cleavage failure established centrosome amplification in transformed cell populations. Tetraploid HCT116 p53(-/-) cells eventually all failed cleavage repeatedly and ceased proliferating. HeLa cells all died or arrested within four cell cycles. Chinese hamster ovary cells proliferated after cleavage failure, but five rounds of induced cleavage failure produced a modest increase in the incidence of centrosome amplification in the short term, which did not rise with more cycles of cleavage failure. This incidence dropped to close to control values in the long term despite a 2-6% rate of spontaneous cleavage failure in the progeny of tetraploid cells.

Rights and Permissions

Citation: J Cell Biol. 2011 Jul 25;194(2):199-207. Link to article on publisher's site

Comments

© 2011 Krzywicka-Racka and Sluder. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

Related Resources

Link to Article in PubMed

PubMed ID

21788368

Included in

Cell Biology Commons

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