A simple enzymatic method for parietal yolk sac removal in early postimplantation mouse embryos
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2007-02-05Keywords
AnimalsCollagenases
Embryonic Development
Endopeptidases
Enzymes
Fluorescent Antibody Technique
Hyaluronoglucosaminidase
In Situ Hybridization
Mice
Microdissection
Yolk Sac
Cell Biology
Metadata
Show full item recordAbstract
Crucial aspects of axial development in mice occur at early postimplantation stages from the time of implantation to the appearance of the primitive streak. However, this period of development is notoriously refractory to experimental approaches due to the small size of the conceptus and to the presence of the parietal yolk sac, a protective tripartite membrane that surrounds the developing egg cylinder. Here, we describe a method that combines enzymatic digestion and mechanical manipulation to remove the parietal yolk sac of conceptuses at stages between 5.5 and 6.5 days post coitum. This method, which is compatible with whole-mount in situ hybridization and immunostaining techniques, offers a significant improvement over conventional dissection techniques, and it will greatly facilitate research in early mammalian development.Source
Dev Dyn. 2007 Feb;236(2):489-93. Link to article on publisher's siteDOI
10.1002/dvdy.21034Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48788PubMed ID
17143861Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/dvdy.21034