Goosecoid and HNF-3beta genetically interact to regulate neural tube patterning during mouse embryogenesis
Authors
Filosa, StefaniaRivera-Pérez, Jaime A.
Gomez, Aitana Perea
Gansmuller, Anne
Sasaki, Hitoshi
Behringer, Richard R.
Ang, Siew-Lan
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
1997-07-01Keywords
AnimalsBody Patterning
DNA-Binding Proteins
Digestive System
Fibroblast Growth Factor 8
*Fibroblast Growth Factors
Gene Expression Regulation, Developmental
Genes, Homeobox
Gestational Age
Goosecoid Protein
Growth Substances
Hedgehog Proteins
Hepatocyte Nuclear Factor 3-beta
Heterozygote
Homeodomain Proteins
In Situ Hybridization
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Mutation
Nervous System
Notochord
Nuclear Proteins
Prosencephalon
Proteins
RNA, Messenger
*Repressor Proteins
*Trans-Activators
*Transcription Factors
Cell Biology
Metadata
Show full item recordAbstract
The homeobox gene goosecoid (gsc) and the winged-helix gene Hepatic Nuclear Factor-3beta (HNF-3beta) are co-expressed in all three germ layers in the anterior primitive streak and at the rostral end of mouse embryos during gastrulation. In this paper, we have tested the possibility of functional synergism or redundancy between these two genes during embryogenesis by generating double-mutant mice for gsc and HNF-3beta. Double-mutant embryos of genotype gsc(-/-);HNF-3beta(+/-) show a new phenotype as early as embryonic days 8.75. Loss of Sonic hedgehog (Shh) and HNF-3beta expression was observed in the notochord and ventral neural tube of these embryos. These results indicate that gsc and HNF-3beta interact to regulate Shh expression and consequently dorsal-ventral patterning in the neural tube. In the forebrain of the mutant embryos, severe growth defects and absence of optic vesicles could involve loss of expression of fibroblast growth factor-8, in addition to Shh. Our results also suggest that interaction between gsc and HNF-3beta regulates other signalling molecules required for proper development of the foregut, branchial arches and heart.Source
Development. 1997 Jul;124(14):2843-54. Link to article on publisher's websitePermanent Link to this Item
http://hdl.handle.net/20.500.14038/48775PubMed ID
9226455Related Resources
Link to Article in PubMedCollections
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