Activation of CD4 cells by fibronectin and anti-CD3 antibody. A synergistic effect mediated by the VLA-5 fibronectin receptor complex

UMMS Affiliation

Department of Medicine, Division of Rheumatology



Document Type


Medical Subject Headings

Antibodies, Monoclonal; Antigens, CD29; Antigens, CD3; Antigens, Differentiation; Antigens, Differentiation, T-Lymphocyte; CD4-Positive T-Lymphocytes; Cells, Cultured; Electrophoresis, Gel, Two-Dimensional; Extracellular Matrix; Fibronectins; Humans; In Vitro Techniques; *Lymphocyte Activation; Receptors, Antigen, T-Cell; Receptors, Cytoadhesin; Receptors, Very Late Antigen


Immunology and Infectious Disease | Musculoskeletal Diseases | Rheumatology | Skin and Connective Tissue Diseases


In this study, fibronectin synergized with anti-CD3 antibody to promote CD4 cell proliferation in a serum-free culture system. The cell-adhesive domain plus additional regions of the fibronectin molecule are involved in this synergy. Anti4B4(CDw29) antibody blocked the activation of CD4 cells in this system. Furthermore, it is the VLA-5 protein within the set of molecules recognized by anti-4B4 that serves as a fibronectin receptor on the CD4 lymphocytes. The VLA-5 fibronectin receptor was mainly expressed on CD4+ CD45R-CDw29+ cells and may in part contribute to the unique function of these cells.

Rights and Permissions

Citation: J Exp Med. 1989 Oct 1;170(4):1133-48.


At the time of publication, Jonathan Kay was not yet affiliated with the University of Massachusetts Medical School.

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