Title

Separation of functionally distinct subpopulations of Corynebacterium parvum-activated macrophages with predominantly stimulatory or suppressive effect on the cell-mediated cytotoxic T cell response

UMMS Affiliation

Department of Medicine, Division of Rheumatology

Date

1-1-1979

Document Type

Article

Medical Subject Headings

Animals; Ascitic Fluid; Cell Adhesion; Cell Separation; *Cytotoxicity, Immunologic; Female; Immunity, Cellular; *Immunosuppression; Leukemia, Experimental; Macrophages; Male; Mice; Mice, Inbred CBA; Propionibacterium acnes; Spleen; T-Lymphocytes; Time Factors

Disciplines

Immunology and Infectious Disease

Abstract

Peritoneal cells (PEC) from mice injected ip with Corynebacterium parvum (CP) showed greatly enhanced suppressive activity on the growth of syngeneic tumor cells and on the generation of alloreactive cytotoxic T lymphocytes (CTL) in vitro. On the other hand, CP-activated PEC exhibited increased immunostimulatory (accessory or A cell) activity as measured by the restoration of the CTL response of nonadherent spleen cells. After fractionation of the CP-activated PEC according to cell size by velocity sedimentation, the mutually antagonistic A cell and immunosuppressive activities were clearly separated and found to be associated with functionally distinct subpopulations of macrophages. Thus A cell function was detected in fractions rich in small and medium sized macrophages which were probably derived from recently arrived monocytes. Immunosuppressive (and anti-tumor) activity was associated with the largest macrophages which were almost devoid of A cell function and probably represented a highly activated and differentiated macrophage subpopulation.

Rights and Permissions

Citation: Cell Immunol. 1979 Jan;42(1):28-41. doi:10.1016/0008-8749(79)90218-1 Link to article on publisher's website

Comments

At the time of publication, Jonathan Kay was not yet affiliated with the University of Massachusetts Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

312146