Title

Imaging the pancreatic vasculature in diabetes models

UMMS Affiliation

Department of Radiology; Program in Molecular Medicine

Publication Date

11-10-2011

Document Type

Article

Subjects

Animals; Diabetes Mellitus, Experimental; Fluorescein-5-isothiocyanate; Gadolinium DTPA; Magnetic Resonance Imaging; Mice; Pancreas; Rats

Disciplines

Endocrine System | Endocrine System Diseases | Investigative Techniques | Radiology

Abstract

BACKGROUND: Vascular parameters, such as vascular volume, flow, and permeability, are important disease biomarkers for both type 1 and type 2 diabetes. Therefore, it is essential to develop approaches to monitor the changes in pancreatic microvasculature non-invasively.

METHODS: Here, we describe the application of the long-circulating, paramagnetic T1 contrast agent, protected Graft Copolymer bearing covalently linked gadolinium diethylenetriaminepentaacetic acid residues and labelled with fluorescein (PGC-GdDTPA-F) for the non-invasive semi-quantitative evaluation of vascular changes in diabetic models using magnetic resonance imaging.

RESULTS: We observed a significantly higher accumulation of protected graft copolymer bearing covalently linked gadolinium diethylenetriaminepentaacetic acid residues and labelled with fluorescein in the pancreata of BBDR rats induced to develop diabetes, as compared to non-diabetic controls at 1 h post-injection. No differences were seen in the blood pool, kidney, or muscle, indicating that the effect is specific to the diabetic pancreas. Fluorescence microscopy revealed a marked increase in contrast agent availability in the pancreas with the development of the pathology. Similar changes were noted in the homozygous Leprdb mouse model of type 2 diabetes. This effect appeared to result both from the increase of vascular volume and permeability.

CONCLUSIONS: High-molecular weight paramagnetic blood volume contrast agents are valuable for the in vivo definition of pancreatic microvasculature dynamics by magnetic resonance imaging. The increase in vascular volume and permeability, associated with diabetic inflammation, can be monitored non-invasively and semi-quantitatively by magnetic resonance imaging in diabetic BBDR rats. This imaging strategy represents a valuable research tool for better understanding of the pathologic process.

Rights and Permissions

Citation: Diabetes Metab Res Rev. 2011 Nov;27(8):767-72. doi: 10.1002/dmrr.1249. Link to article on publisher's site

Journal/Book/Conference Title

Diabetes/metabolism research and reviews

Related Resources

Link to Article in PubMed

PubMed ID

22069257