Title

Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle

UMMS Affiliation

Department of Radiology

Publication Date

7-21-2010

Document Type

Article

Subjects

Animals; Carbocyanines; Cell Line, Tumor; Cell Nucleus; Female; Fluorescence; Heterocyclic Compounds, 1-Ring; Humans; Mice; Mice, Nude; Nanoparticles; Neoplasms; Organometallic Compounds; Streptavidin; Xenograft Model Antitumor Assays

Disciplines

Medicinal-Pharmaceutical Chemistry | Nanomedicine | Neoplasms | Oncology | Radiology

Abstract

Combining two or more different imaging modalities in the same agent can be of considerable value in molecular imaging. We describe the use of streptavidin nanoparticle-based complexes as multimodality imaging agents to achieve tumor detection in a mouse model by both fluorescence and nuclear imaging. Up to four biotinylated functionalities can be readily attached to these streptavidin nanoparticles without apparent influence on their properties and with reasonable pharmacokinetics and therefore may be ideally suited for multimodality imaging. By binding a biotinylated anti-Her2 Herceptin antibody to provide tumor targeting, a biotinylated DOTA chelator labeled with (111)ln and a biotinylated Cy5.5 fluorophore to a streptavidin nanoparticle, we demonstrated multimodality imaging in SUM190 (Her2+) tumor bearing mice on both an IVIS fluorescence camera and a NanoSPECT/CT small animal nuclear camera. The imaging results show high tumor accumulation and strong tumor-to-normal tissue contrast by both fluorescence and nuclear imaging. The subsequent biodistribution study confirmed the specific tumor accumulation in that tumor accumulation of radioactivity at 40 h was 21 ID%/g and therefore much higher than all other tissues including liver, heart, kidney, spleen, and muscle that accumulated 8.7, 2.5, 6.9, 7.2, and 1.9 ID%/g, respectively. In conclusion, the streptavidin nanoparticle under development in this laboratory was used effectively for multimodality imaging of tumor in mice by fluorescence and nuclear detection. Presumably, other imaging modalities could also be considered.

Rights and Permissions

Citation: Bioconjug Chem. 2010 Jul 21;21(7):1385-8. doi: 10.1021/bc100081h. Link to article on publisher's site

Journal/Book/Conference Title

Bioconjugate chemistry

Related Resources

Link to Article in PubMed

PubMed ID

20557066