Title

(99m)Tc-MORF oligomers specific for bacterial ribosomal RNA as potential specific infection imaging agents

UMMS Affiliation

Department of Radiology, Division of Nuclear Medicine

Date

11-1-2013

Document Type

Article

Medical Subject Headings

Animals; Bacterial Infections; Escherichia coli; Half-Life; In Situ Hybridization, Fluorescence; Klebsiella pneumoniae; Mice; Microscopy, Fluorescence; Morpholinos; Organotechnetium Compounds; RNA, Bacterial; RNA, Ribosomal, 16S; Radiopharmaceuticals; Staphylococcus aureus; Tissue Distribution; Tomography, Emission-Computed, Single-Photon; Tomography, X-Ray Computed

Disciplines

Bacteria | Bacterial Infections and Mycoses | Medicinal and Pharmaceutical Chemistry | Medicinal-Pharmaceutical Chemistry | Radiology

Abstract

PURPOSE: Radiolabeled oligomers complementary to the 16S rRNA in bacteria were investigated as bacterial infection imaging agents.

METHODS AND RESULTS: Identical sequences with backbones phosphorodiamidate morpholino (MORF), peptide nucleic acid (PNA), and phosphorothioate DNA (PS-DNA) were (99m)Tc-labeled and evaluated for binding to bacterial RNA. MORF binding to RNA from Escherichia coli strains SM101 and K12 was 4- and 150-fold higher compared to PNA and PS-DNA, respectively. Subsequently MORF oligomer in fluorescence in situ hybridization showed a stronger signal with study MORF compared to control in fixed preparations of two E. coli strains and Klebsiella pneumoniae. Flow cytometry analysis showed study MORF accumulation to be 8- and 80-fold higher compared to the control in live K. pneumoniae and Staphylococcus aureus, respectively. Further, fluorescence microscopy showed increased accumulation of study MORF over control in live E. coli and K. pneumonia. Binding of (99m)Tc-study MORF to RNA from E. coli SM101 and K12 was 30.4 and 117.8pmol, respectively, per 10(10) cells. Mice with K. pneumoniae live or heat-killed (sterile inflammation) in one thigh at 90min for both (99m)Tc-study MORF and control showed higher accumulation in target thighs than in blood and all other organs expect for kidneys and small intestine. Accumulation of (99m)Tc-study MORF was significantly higher (p = 0.009) than that of the control in the thigh with sterile inflammation.

CONCLUSION: A (99m)Tc-MORF oligomer complimentary to the bacterial 16S rRNA demonstrated binding to bacterial RNA in vitro with specific accumulation into live bacteria. Radiolabeled MORF oligomers antisense to the bacterial rRNA may be useful to image bacterial infection.

Rights and Permissions

Citation: Bioorg Med Chem. 2013 Nov 1;21(21):6523-30. doi: 10.1016/j.bmc.2013.08.034. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Keywords

MORF, PNA, PS-DNA, bacterial infection imaging, SPECT/CT

PubMed ID

24054488