Finding the striatum in sheep: use of a multi-modal guided approach for convection enhanced delivery
Department of Radiology; Department of Neurosurgery; Department of Microbiology and Physiological Systems; Department of Medicine; Department of Animal Medicine; Department of Neurology; Department of Pediatrics, Division of Pediatric Pulmonology; Gene Therapy Center
Animal Sciences | Nervous System Diseases | Radiology | Therapeutics
Our goal is delivery of a long-term treatment for Huntington's disease. We administer intracerebrally in sheep adeno-associated virus (AAV) to establish optimal safety, spread and neuronal uptake of AAV based therapeutics. Sheep have large gyrencephalic brains and offer the opportunity to study a transgenic Huntington's disease model. However, lack of a relevant brain stereotactic atlas and the difficulty of skull fixation make conventional stereotaxy unreliable. We describe a multi-modal image-guidance technique to achieve accurate placement of therapeutics into the sheep striatum.
Sheep brain, huntingtin therapeutics, stereotaxis placement
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Citation: J Huntingtons Dis. 2013;2(1):41-5. doi: 10.3233/JHD-130053. Link to article on publisher's site
Journal of Huntington's disease
van der Bom, I. M. J.; Moser, Richard P.; Gao, Guangping; Mondo, Erica; O'Connell, Denice; Gounis, Matthew J.; McGowan, Sarah; Chaurette, J.; Bishop, N.; Sena-Esteves, Miguel; Mueller, Christian; and Aronin, Neil, "Finding the striatum in sheep: use of a multi-modal guided approach for convection enhanced delivery" (2013). Radiology Publications and Presentations. 287.