Assessing antibody pharmacokinetics in mice with in vivo imaging
Department of Radiology
Medical Subject Headings
Animals; Antibodies; Diagnostic Imaging; Fluorescence; Image Processing, Computer-Assisted; Luminescence; Mice; Pharmacokinetics; Physics; Positron-Emission Tomography; Radiopharmaceuticals; Tomography, Emission-Computed, Single-Photon
Medical Pharmacology | Medicinal and Pharmaceutical Chemistry | Medicinal Chemistry and Pharmaceutics | Radiochemistry | Radiology | Therapeutics
Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.
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Citation: J Pharmacol Exp Ther. 2011 May;337(2):350-8. doi: 10.1124/jpet.110.172916. Epub 2011 Feb 11. Link to article on publisher's site.
Hoppin, Jack; Orcutt, Kelly Davis; Hesterman, Jacob Y.; Silva, Matthew D.; Cheng, Dengfeng; Lackas, Christian; and Rusckowski, Mary, "Assessing antibody pharmacokinetics in mice with in vivo imaging" (2011). Radiology Publications and Presentations. 107.