Title

Assessing antibody pharmacokinetics in mice with in vivo imaging

UMMS Affiliation

Department of Radiology

Date

5-1-2011

Document Type

Article

Medical Subject Headings

Animals; Antibodies; Diagnostic Imaging; Fluorescence; Image Processing, Computer-Assisted; Luminescence; Mice; Pharmacokinetics; Physics; Positron-Emission Tomography; Radiopharmaceuticals; Tomography, Emission-Computed, Single-Photon

Disciplines

Medical Pharmacology | Medicinal and Pharmaceutical Chemistry | Medicinal Chemistry and Pharmaceutics | Radiochemistry | Radiology | Therapeutics

Abstract

Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.

Rights and Permissions

Citation: J Pharmacol Exp Ther. 2011 May;337(2):350-8. doi: 10.1124/jpet.110.172916. Epub 2011 Feb 11. Link to article on publisher's site.

Related Resources

Link to Article in PubMed

PubMed ID

21317355