Stability of interferon-gamma and interleukin-10 responses to Plasmodium falciparum liver stage antigen 1 and thrombospondin-related adhesive protein immunodominant epitopes in a highland population from Western Kenya
Department of Quantitative Health Sciences; Department of Pediatrics
Medical Subject Headings
Adolescent; Adult; Animals; Antigens, Protozoan; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Gene Expression Regulation; Humans; Immunodominant Epitopes; Interferon-gamma; Interleukin-10; Kenya; Leukocytes, Mononuclear; Malaria, Falciparum; Parasitemia; Plasmodium falciparum; Prevalence; Protozoan Proteins; Time Factors; Young Adult
Biostatistics | Epidemiology | Health Services Research | Immunology and Infectious Disease | Pediatrics
Long-term planning to prevent malaria epidemics requires in-depth understanding of immunity to Plasmodium falciparum in areas of unstable transmission. Cytokine responses to immunodominant epitope peptides from liver stage antigen 1 (LSA-1) and thrombospondin-related adhesive protein (TRAP) were evaluated over a nine-month interval in adults and children in Kenya from a malaria epidemic-prone highland area after several years of low transmission. The proportion and magnitude of interferon-gamma ELISPOT responses and the proportion of interleukin-10 responders to LSA-1 and TRAP peptides tended to be higher in adults than children. Frequencies of interferon-gamma responders to these peptides were similar at the two time points, but responses were not consistently generated by the same persons. These results suggest that T cell memory to pre-erythrocytic stage malaria antigens is maintained but may be unavailable for consistent detection in peripheral blood, and that these antigens induce both pro-inflammatory and anti-inflammatory cytokine responses in this population.
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Citation: Am J Trop Med Hyg. 2009 Sep;81(3):489-95. Link to article on publisher's site