Kinetics of Cryptosporidium parvum-specific cytokine responses in healing and nonhealing murine models of C. parvum infection
Department of Medicine, Division of Preventive and Behavioral Medicine
Animals; Antigens, Protozoan; Body Weight; Cryptosporidiosis; Cryptosporidium parvum; Cytokines; Disease Models, Animal; Humans; Kinetics; Lymphocyte Activation; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Species Specificity; T-Lymphocyte Subsets
Immunology and Infectious Disease | Parasitology
Susceptibility or resistance to infection with Cryptosporidium parvum correlates with the ability of mice to produce characteristic panels of cytokines in response to infection. Both adult healing and nonhealing mouse models of cryptosporidiosis were used to study the cell-mediated immune response during the course of C. parvum infection. Mesenteric lymph node (MLN) lymphocytes from both mouse models were proliferated after ex vivo re-stimulation with C. parvum sporozoite antigen. Study of the cytokine profile from the supernatant of proliferated MLN cells revealed that healing mice produced greater levels of Th1 (IFN-gamma and IL-2) and moderate amounts of Th2 (IL-4, IL-5, IL-6, and IL-10) cytokines throughout the course of infection. Whereas, MLN cells from nonhealing mice produced no IFN-gamma, low levels of IL-2 and IL-4, and higher levels of IL-5, IL-6, and IL-10 cytokines. These results suggest that the capacity to produce both Th1 and Th2 cytokines, rather than the presence of Th2 cytokines alone, determines the effective immune response against C. parvum infection.
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Citation: Parasitol Res. 2005 Oct;97(4):309-17. Epub 2005 Jul 29. Link to article on publisher's site