HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation
Authors
Rosa, AnnachiaraChande, Ajit
Ziglio, Serena
De Sanctis, Veronica
Bertorelli, Roberto
Goh, Shih Lin
McCauley, Sean M.
Nowosielska, Anetta
Antonarakis, Stylianos E.
Luban, Jeremy
Santoni, Federico Andrea
Pizzato, Massimo
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2015-10-08Keywords
AnimalsCell Line
Cell Membrane
Endosomes
Evolution, Molecular
Gene Products, gag
Gene Products, nef
HIV-1
Host Specificity
*Host-Pathogen Interactions
Humans
Leukemia Virus, Murine
Membrane Proteins
Neoplasm Proteins
Primates
Receptors, Cell Surface
Virion
nef Gene Products, Human Immunodeficiency Virus
rab GTP-Binding Proteins
Biochemistry
Molecular Biology
Metadata
Show full item recordAbstract
HIV-1 Nef, a protein important for the development of AIDS, has well-characterized effects on host membrane trafficking and receptor downregulation. By an unidentified mechanism, Nef increases the intrinsic infectivity of HIV-1 virions in a host-cell-dependent manner. Here we identify the host transmembrane protein SERINC5, and to a lesser extent SERINC3, as a potent inhibitor of HIV-1 particle infectivity that is counteracted by Nef. SERINC5 localizes to the plasma membrane, where it is efficiently incorporated into budding HIV-1 virions and impairs subsequent virion penetration of susceptible target cells. Nef redirects SERINC5 to a Rab7-positive endosomal compartment and thereby excludes it from HIV-1 particles. The ability to counteract SERINC5 was conserved in Nef encoded by diverse primate immunodeficiency viruses, as well as in the structurally unrelated glycosylated Gag from murine leukaemia virus. These examples of functional conservation and convergent evolution emphasize the fundamental importance of SERINC5 as a potent anti-retroviral factor.Source
Nature. 2015 Oct 8;526(7572):212-7. doi: 10.1038/nature15399. Epub 2015 Sep 30. Link to article on publisher's siteDOI
10.1038/nature15399Permanent Link to this Item
http://hdl.handle.net/20.500.14038/44462PubMed ID
26416734Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/nature15399