Title

Adenosine enhances nitric oxide production by vascular endothelial cells

UMMS Affiliation

Department of Physiology; Department of Surgery

Date

8-1995

Document Type

Article

Subjects

Adenosine; Animals; Arginine; Carotid Arteries; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular; Humans; Nitric Oxide; Saphenous Vein; Swine; Theophylline; omega-N-Methylarginine

Disciplines

Physiology | Surgery

Abstract

Adenosine per se is a potent vasodilator of vascular smooth muscle. Endothelial cells modulate vascular tone via the release of nitric oxide (NO), which also elicits vasodilation. This study was undertaken to determine whether adenosine could directly stimulate endothelial cells to enhance NO production, which could subsequently reduce vascular tone. NO production was evaluated in porcine carotid artery endothelial cells (PCAEC) and human saphenous vein endothelial cells (HSVEC) seeded on multiwell plates, grown to confluence, and treated with adenosine for 1 h. The bathing medium was collected, and the NO production was determined as reflected by the formation of NO2- and NO3-. NO production by PCAEC was significantly increased by adenosine in a dose-dependent manner, whereas there was only an insignificant tendency for an increase by HSVEC. The addition of the NO synthase competitive inhibitor, NG-monomethyl-L-arginine (NMMA), or the adenosine receptor antagonist, theophylline, prevented the increase in NO production by adenosine. The results suggest that adenosine stimulates, by a receptor-mediated mechanism, the production of NO by arterial, but not by venous, endothelial cells.

Rights and Permissions

Citation: Am J Physiol. 1995 Aug;269(2 Pt 1):C519-23.

Related Resources

Link to article in PubMed

PubMed ID

7653535