Title
Anoxia-induced changes in purine nucleoside metabolism of in vitro aged human fibroblasts
UMMS Affiliation
Department of Physiology
Date
2-8-2002
Document Type
Article
Subjects
Adenosine; Cell Aging; Cell Hypoxia; Cell Line; Chromatography, High Pressure Liquid; Fibroblasts; Humans; Inosine; Lung; Time Factors
Disciplines
Cellular and Molecular Physiology | Physiology
Abstract
Inosine deriving from the metabolism of adenosine or inosine monophosphate (IMP) in the fibroblast provides the substrate for xanthine oxidase and is, therefore, an important source of toxic oxygen free radicals. With well-oxygenated medium, adenosine release appears to be greater for aged than young fibroblasts. In that the adenosine release by young cells is enhanced by reduced oxygenation, the effect anoxic stress on the release of the purine nucleosides adenosine and inosine by low-passage (PDL 23-26; young) vs. high-passage (PDL 43-51; aged) human lung fibroblasts (IMR-90) was studied. Cultures of confluent fibroblasts were incubated for 16 hr under normoxic (NF) or anoxic (AF) atmospheres. The release of adenosine and inosine was determined by HPLC at 0, 3, 6 and 24 hr after termination of the 16-hr period. Immediately following anoxia (time 0), adenosine release by young AF was 29% greater than for young NF, whereas both the youn
Rights and Permissions
Citation: Life Sci. 2002 Feb 8;70(12):1369-82.
Related Resources
PubMed ID
11883713