Title

Adenosine stimulates proliferation of human endothelial cells in culture

UMMS Affiliation

Department of Physiology; Department of Medicine, Division of Pulmonary, Allergy and Critical Care

Date

7-1993

Document Type

Article

Subjects

Adenosine; Adenosine Deaminase; Cell Division; Cells, Cultured; Endothelium, Vascular; Humans; Receptors, Purinergic; Theophylline; Thymidine

Disciplines

Cellular and Molecular Physiology | Physiology

Abstract

The effect of adenosine on proliferation of human endothelial cells was investigated by adding adenosine to the medium of cultures derived from human umbilical veins. Cell counts on cultures grown in 10 microM adenosine for 4-7 days were 41-53% greater than counts from control cultures. In contrast, 10 microM adenosine had no effect on growth of a human fibroblast cell strain (IMR-90). Neither inosine nor 2',5'-dideoxyadenosine influenced endothelial cell growth at concentrations of 0.1 or 10 microM. Addition of adenosine deaminase abolished the proliferative effect of added adenosine and inhibited proliferation by 16% in control cultures, suggesting that endogenous adenosine may enhance proliferation in culture. The adenosine receptor antagonist, 8-phenyltheophylline, at 0.1 and 1.0 microM blocked the enhanced proliferation caused by 10 microM adenosine. Addition of 10 microM adenosine enhanced DNA synthesis in endothelial cell cultures as indicated by an increased incorporation of [3H]thymidine into acid-insoluble cell material. The results indicate that addition of physiological concentrations of adenosine to human umbilical vein endothelial cell cultures stimulates proliferation, possibly via a surface receptor, and suggest that adenosine may be a factor for human endothelial cell growth and possibly angiogenesis.

Rights and Permissions

Citation: Am J Physiol. 1993 Jul;265(1 Pt 2):H131-8.

Related Resources

Link to article in PubMed

PubMed ID

8342624